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Streptomyces venezuelae ATCC 10712

ABSTRACT: ChIP-Seq data of the Streptomyces venezualae protein Sven2756 (MtrA) over a time course of 10h, 12h, 14h, 16h, 18h and 20h.

PROVIDER: PRJNA328802 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3880060.fastq.gz	Fastqsanger.gz
	SRR3880061.fastq.gz	Fastqsanger.gz
	SRR3880062.fastq.gz	Fastqsanger.gz
	SRR3880063.fastq.gz	Fastqsanger.gz
	SRR3880064.fastq.gz	Fastqsanger.gz

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ChIP-Seq data of the Streptomyces venezualae protein Sven2756 (MtrA) over a time course of 10h, 12h, 14h, 16h, 18h and 20h.

Project description:We report the results of ChIP-Seq experiment investigating the MtrA TCS response regulator. The experiment utilised an in trans copy of mtrA with an N-terminal 3xFlag tag encoded on the plasmid pMS82 (containing a phiBT1 integration site). The experiment utilised beads associated with anti-flag antibodies, specific for the Flag tagged protein. We report important targets involved in cell division and salt stress with key findings supporting the hypothesis that MtrA is essential. Samples were taken over a time course of 10h, 12h, 14h, 16h, 18h and 20h.

2017-07-12 | GSE84311 | GEO

S. coelicolor M145 Time-course Study in R5- medium (Culture# 3)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M145 wild type and disruption mutants of regulatory genes (afsS and absA1) known to affect antibiotic biosynthesis. Keywords: Time course The time-course study involved analysis of 13 samples (10h, 13h, 14h, 16h, 18h, 20h, 22h, 24h, 27h, 29h, 32h, 34h, 37h). Of these, 13h, 14h, 16h, 18h, 20h, 22h, 24h, 32h, and 37h sample were analyzed on duplicate arrays (2 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

2010-06-11 | E-GEOD-8107 | biostudies-arrayexpress

Streptomyces venezuelae ATCC 10712

Project description:ChIP-Seq data of the Streptomyces venezualae protein Sven6563 (RsrR)

| PRJNA320415 | ENA

Midgut metamorphosis timecourse

Project description:A midgut reference sample is compared to midgut samples taken at various stages before (18h, 4h), at (0h), and after (2h, 3h, 4h, 5h, 6h, 8h, 10h, 12h) puparium formation. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other

2003-07-23 | GSE543 | GEO

Streptomyces venezuelae ATCC 10712

Project description:dRNA-seq data of the Streptomyces venezualae WT and mutant (sven6563::apr (rsrR::apr)) strains at time point 16 h

| PRJNA320533 | ENA

Streptomyces venezuelae ATCC 10712

Project description:RNA-seq data of the Streptomyces venezualae WT and mtrB::apr mutant strains at time point 14 and 20 h (biological duplicates).

| PRJNA345635 | ENA

RNA-seq data of the Streptomyces venezualae WT and mtrB::apr mutant strains at time point 14 and 20 h (biological duplicates).

Project description:We report here the sequence results of S. venezualae WT and the mutant mtrB::apr strains at 14h and 20h and the differential expression profile of WT vs. Mutant.

2017-10-06 | GSE87728 | GEO

New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712.

Project description:Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916-sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does not produce chloramphenicol. Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na(+)/H(+) antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway.

| S-EPMC4249514 | biostudies-literature

Effect of ZC3H28 depletion (RNAi) in Trypanosoma brucei bloodstream forms

Project description:Bloodstream-form trypanosomes with inducible RNAi targeting ZC3H28 were incubated with the tetracycline inducer for 10h, cells without tetracycline were used as controls. This experiment was done in triplicates. In another similar experiment, bloodstream-form trypanosomes with inducible RNAi targeting ZC3H28 were incubated with the tetracycline inducer for 14h, 16h, and 24h. Cells without tetracycline (named ''no tet'' or ''0h'') were used as controls. This experiment was done in duplicates.

2021-07-31 | E-MTAB-10751 | biostudies-arrayexpress

Influence of core divisome proteins on cell division in Streptomyces venezuelae ATCC 10712.

Project description:The sporulating, filamentous soil bacterium Streptomyces venezuelae ATCC 10712 differentiates under submerged and surface growth conditions. In order to lay a solid foundation for the study of development-associated division for this organism, a congenic set of mutants was isolated, individually deleted for a gene encoding either a cytoplasmic (i.e. ftsZ) or core inner membrane (i.e. divIC, ftsL, ftsI, ftsQ, ftsW) component of the divisome. While ftsZ mutants are completely blocked for division, single mutants in the other core divisome genes resulted in partial, yet similar, blocks in sporulation septum formation. Double and triple mutants for core divisome membrane components displayed phenotypes that were similar to those of the single mutants, demonstrating that the phenotypes were not synergistic. Division in this organism is still partially functional without multiple core divisome proteins, suggesting that perhaps other unknown lineage-specific proteins perform redundant functions. In addition, by isolating an ftsZ2p mutant with an altered -10 region, the conserved developmentally controlled promoter was also shown to be required for sporulation-associated division. Finally, microscopic observation of FtsZ-YFP dynamics in the different mutant backgrounds led to the conclusion that the initial assembly of regular Z rings does not per se require the tested divisome membrane proteins, but the stability of Z rings is dependent on the divisome membrane components tested. The observation is consistent with the interpretation that Z ring instability likely results from and further contributes to the observed defects in sporulation septation in mutants lacking core divisome proteins.

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