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Streptomyces venezuelae ATCC 10712

ABSTRACT: ChIP-Seq data of the Streptomyces venezualae protein Sven6563 (RsrR)

PROVIDER: PRJNA320415 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR3472910.fastq.gz	Fastqsanger.gz
	SRR3472911.fastq.gz	Fastqsanger.gz
	SRR3472912.fastq.gz	Fastqsanger.gz
	SRR3472909.fastq.gz	Fastqsanger.gz

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Streptomyces venezuelae

Project description:ChIP-exo data of the Streptomyces venezualae protein Sven6563 (RsrR)

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Streptomyces venezuelae ATCC 10712

Project description:Streptomyces venezuelae RsrR ChIP-seq

| PRJNA315371 | ENA

Streptomyces venezuelae ATCC 10712

Project description:dRNA-seq data of the Streptomyces venezualae WT and mutant (sven6563::apr (rsrR::apr)) strains at time point 16 h

| PRJNA320533 | ENA

Streptomyces venezuelae ATCC 10712

Project description:ChIP-Seq data of the Streptomyces venezualae protein Sven2756 (MtrA) over a time course of 10h, 12h, 14h, 16h, 18h and 20h.

| PRJNA328802 | ENA

Streptomyces venezuelae ATCC 10712

Project description:RNA-seq data of the Streptomyces venezualae WT and mtrB::apr mutant strains at time point 14 and 20 h (biological duplicates).

| PRJNA345635 | ENA

New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712.

Project description:Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916-sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does not produce chloramphenicol. Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na(+)/H(+) antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway.

| S-EPMC4249514 | biostudies-literature

Influence of core divisome proteins on cell division in Streptomyces venezuelae ATCC 10712.

Project description:The sporulating, filamentous soil bacterium Streptomyces venezuelae ATCC 10712 differentiates under submerged and surface growth conditions. In order to lay a solid foundation for the study of development-associated division for this organism, a congenic set of mutants was isolated, individually deleted for a gene encoding either a cytoplasmic (i.e. ftsZ) or core inner membrane (i.e. divIC, ftsL, ftsI, ftsQ, ftsW) component of the divisome. While ftsZ mutants are completely blocked for division, single mutants in the other core divisome genes resulted in partial, yet similar, blocks in sporulation septum formation. Double and triple mutants for core divisome membrane components displayed phenotypes that were similar to those of the single mutants, demonstrating that the phenotypes were not synergistic. Division in this organism is still partially functional without multiple core divisome proteins, suggesting that perhaps other unknown lineage-specific proteins perform redundant functions. In addition, by isolating an ftsZ2p mutant with an altered -10 region, the conserved developmentally controlled promoter was also shown to be required for sporulation-associated division. Finally, microscopic observation of FtsZ-YFP dynamics in the different mutant backgrounds led to the conclusion that the initial assembly of regular Z rings does not per se require the tested divisome membrane proteins, but the stability of Z rings is dependent on the divisome membrane components tested. The observation is consistent with the interpretation that Z ring instability likely results from and further contributes to the observed defects in sporulation septation in mutants lacking core divisome proteins.

| S-EPMC8131025 | biostudies-literature

Streptomyces venezuelae ATCC 10712

Project description:Streptomyces venezuelae ATCC 10712 genome sequencing project

| PRJNA62209 | ENA

Streptomyces venezuelae ATCC 10712

Project description:Streptomyces exploration is triggered by fungal interactions and volatile signals

| PRJNA341574 | ENA

Determination by ChIP-chip of the binding sites of the developmental transcription regulator BldC in the genome of Streptomyces venezuelae ATCC 10712

Project description:BldC is a transcriptional regulator essential for morphological development Streptomyces venezuelae. Although bldC deletion strain is unable to produce aerial hyphae, electron microscopy reveals that almost all of the colony biomass is in the form of spores rather than undifferentiated vegetative hyphae. This ChIP-chip experiment was carried out to determine the binding sites, and thence the regulon, of BldC in Streptomyces venezuelae. Cy3(IP):Cy5(Total) signal ratios in the wild type were compared to those in a bldC knockout strain.

2017-11-01 | E-MTAB-6129 | biostudies-arrayexpress

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