An automatec microwell platform for large-scale single cell RNA-seq.
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: GSE85575
PROVIDER: PRJNA338815 | ENA |
REPOSITORIES: ENA
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Project description:We report an automated microwell array platform for single cell RNA-seq with significantly improved performance over previous implementations. We demonstrate cell capture efficiencies of >50%, compatibility with commercially available barcoded mRNA capture beads, and parallel expression profiling from thousands of individual cells. We apply our system to comprehensively assess heterogeneity in gene expression of patient-derived glioma neurospheres and uncover subpopulations similar to those observed in human glioma tissue.
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