Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mosquito hematophage-mediated GABAergic activation facilitates acquisition of mosquito-borne viruses

ABSTRACT: Mosquito hematophage-mediated GABAergic activation facilitates acquisition of mosquito-borne viruses

PROVIDER: PRJNA356718 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5087460.fastq.gz	Fastqsanger.gz
	SRR5087451.fastq.gz	Fastqsanger.gz
	SRR5087461.fastq.gz	Fastqsanger.gz
	SRR5087452.fastq.gz	Fastqsanger.gz
	SRR5087462.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 17

Similar Datasets

Mosquito hematophage-mediated GABAergic activation facilitates acquisition of mosquito-borne viruses

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-12-30 | GSE91034 | GEO

Mosquito hematophage-mediated GABAergic activation facilitates acquisition of mosquito-borne viruses (infection)

Project description:Gene regulation by infection flavivirus, alphavirus and orthobunyavirus in Aedes aegypti

2017-12-30 | GSE91027 | GEO

Mosquito hematophage-mediated GABAergic activation facilitates acquisition of mosquito-borne viruses (dsRNA knocked-down)

Project description:Two independent dsRNAs to knocked-down the AaGABAA-R1 gene in A. aegypti

2017-12-30 | GSE91032 | GEO

Aedes aegypti

Project description:Mosquito hematophage-mediated GABAergic activation facilitates acquisition of mosquito-borne viruses (infection)

| PRJNA356710 | ENA

Aedes aegypti

Project description:Mosquito hematophage-mediated GABAergic activation facilitates acquisition of mosquito-borne viruses (dsRNA knocked-down)

| PRJNA356717 | ENA

Mosquito-borne diseases and Omics: Tissue-restricted expression and alternative splicing revealed by transcriptome profiling of Anopheles stephensi

Project description:Malaria is as one of the most debilitating mosquito-borne global health burdens. While much of the malaria and mosquito-borne disease attention have focused on Africa, South East Asia accounts for a sizable portion of the malaria global burden. Moreover, about 50% of the Asian malaria incidence and deaths have been from India. The completion of genome sequence of Anopheles stephensi, a major malaria vector in Asia, offers new opportunities for global health innovation, not to mention for progress in deciphering the vectorial ability of this mosquito species at a molecular level. Moving forward, tissue-based expression profiling would be the next obvious step in understanding gene functions of Anopheles stephensi. We report here the first study, to the best of our knowledge, on transcriptomic profile of four important organs of an adult female Anopheles stephensi mosquito (midgut, Malpighian tubules, fat body and ovary). In all, we identified over 21,000 transcripts corresponding to more than 12,000 gene loci from these four tissues. This study provides the tissue-based expression profiles of majority of annotated transcripts in Anopheles stephensi genome, and the dynamics of alternative splicing in these tissues. Understanding the transcript expression and gene function at the tissue level would immensely help in enhancing our knowledge of this important vector and decipher the putative role of these tissues. This knowledge might in turn provide the basis of selection of candidates for future studies on vectorial ability and novel molecular targets to intercept malaria transmission.

2017-06-06 | GSE99679 | GEO

Induction and suppression of Haemaphysalis longicornis ticks antiviral RNA interference responses by arthropod-borne RNA viruses (RNA-Seq)

Project description:Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne bunyavirus that causes severe clinical symptoms and mortality in humans. Haemaphysalis longicornis tick has been identified as the competent vector for SFTSV transmission. Although antiviral RNA interference (RNAi) in insects has been well documented, the degree to which RNAi contributes to antiviral defense in ticks is still largely elusive. In this study, utilizing arthropod-borne RNA viruses, including SFTSV, we find abundant virus-derived small interfering RNAs (vsiRNAs) are induced in H. longicornis after infection through either microinjection or natural blood-feeding. Furthermore, we identify a Dicer2-like homolog, the core protein of antiviral RNAi pathway, in H. longicornis and knocking down this gene exacerbated virus amplification. To counteract this antiviral RNAi of ticks, viruses have evolved suppressors of RNAi (VSRs). Here, we show that reduced viral replication inversely correlated with the accumulation of vsiRNAs in ticks after infection with recombinant sindbis virus (SINV) expressing heterologous VSR proteins. Elucidating the antiviral RNAi pathway of ticks by model arthropod-borne RNA viruses in vivo is critical to understanding the virus-host interaction, providing a feasible intervention strategy to control tick-borne arbovirus transmission.

2024-01-16 | GSE165139 | GEO

Differences in Genetic Diversity of Tick-Borne Flaviviruses

Project description:There are very few studies exploring the genetic diversity of tick-borne encephalitis complex viruses. Most of the viruses have been sequenced using capillary electrophoresis, however, very few viruses have been analyzed using deep sequencing to look at the genotypes in each virus population. In this study, different viruses and strains belonging to the tick-borne encephalitis complex were sequenced and genetic diversity was analyzed. Shannon entropy and single nucleotide variants were used to compare the viruses. Then genetic diversity was compared to the phylogenetic relationship of the viruses.

2023-12-04 | E-MTAB-12112 | biostudies-arrayexpress

Insect-Specific Chimeric Viruses Potentiated Antiviral Responses and Inhibited Pathogenic Alphavirus Growth in Mosquito Cells

Project description:Most alphaviruses are transmitted by mosquito vectors and infect a wide range of vertebrate hosts, with a few exceptions. Eilat virus (EILV) in this genus is characterized by a host range restricted to mosquitoes. Its chimeric viruses have been developed as safe and effective vaccine candidates and diagnostic tools. Here, we investigated the interactions between these insect-specific viruses (ISVs) and mosquito cells, unveiling their potential roles in determining vector competence and arbovirus transmission. By RNA sequencing, we found that these ISVs profoundly modified host cell gene expression profiles. Two EILV-based chimeras, consisting of EILV’s nonstructural genes and the structural genes of Chikungunya virus (CHIKV) or Venezuelan equine encephalitis virus (VEEV), namely EILV/CHIKV (E/C) and EILV/VEEV (E/V), induced more intensive transcriptome regulation than parental EILV and activated different antiviral mechanisms in host cells. We demonstrated that E/C robustly promoted antimicrobial peptide production and E/V strongly upregulated the RNA interference pathway components. This also highlighted the intrinsic divergences between CHIKV and VEEV, representatives of the Old World and New World alphaviruses. In contrast, EILV triggered a limited antiviral response. We further showed that initial chimera infections efficiently inhibited subsequent pathogenic alphavirus replication, especially in the case of E/V infection, which almost prevented VEEV and Sindbis virus (SINV) superinfections. Altogether our study provided valuable information on developing ISVs as biological control agents.

2023-01-19 | GSE220562 | GEO

Ae. aegypti dyspepsia (AAEL000471) is involved in mosquito iron homeostasis and is critical for reproductive fitness

Project description:As a key vector for the major arthropod-borne viruses (arboviruses), such as dengue, Zika and chikungunya, control of Aedes aegypti represents a major challenge in public health. Bloodmeal acquisition is necessary for the reproduction of vector mosquitoes and pathogen transmission. Blood contains potentially toxic amounts of iron while it provides nutrients for mosquito offspring; disruption of iron homeostasis in the mosquito may therefore lead to a novel control strategies. We previously described a potential iron exporter in Ae. aegypti after a targeted functional screen of ZIP and ZnT family genes. In this study, we performed an RNAseq-based screen in an Ae. aegypti cell line cultured under iron-deficient and iron-excess conditions. A subset of differentially expressed genes were analyzed via a cytosolic iron-sensitive dual-luciferase reporter assay with several gene candidates potentially involved in iron transport. In vivo gene silencing resulted in significant reduction of fecundity (egg number) and fertility (hatch rate) for one gene, AAEL000471. Consistent with a role in iron homeostasis, ferritin expression in the midgut was reduced upon silencing of AAEL000471. Silencing of AAEL000471 also resulted in delayed/impaired excretion and digestion. Together these studies increase our understanding of gene regulatory networks controlling iron homeostasis in bloodfeeding mosquitoes, and suggest new targets for vector control based on the disruption of this critical process.

2021-04-01 | GSE160498 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data