Project description:The aim of the experiments was to determine the regulon of the Bacillus subtilis alternative sigma factor SigI. Biological relevance: To expand our knowledge about Bacillus subtilis transcriptional network under unfavorable conditions. Experimental workflow overview: Bacillus subtilis 168 trp+ (BaSysBio) was used as the genetic background. (i) sigI-rsgI knock-out, (ii) rsgI knock-out, and (iii) wr strains were cultured in LB medium to mid-exponential phase at 37°C and 52°C. Total RNA was isolated from 3 ml of the culture. rRNA was depleted from the samples with RiboMinus; subsequently RNA-seq libraries were prepared (Illumina compatible NEXTflex Rapid Directional RNA-Seq Kit, Bioo Scientific) and sequenced at the EMBL GeneCore facility. The experiment was performed in three independent replicates.

2018-06-26 | E-MTAB-6314 | biostudies-arrayexpress

Lederbergia wuyishanensis strain:DSM 27848 | isolate:DSM 27848

Project description:Bacillus wuyishanensis DSM 27848 genome sequencing

| PRJNA708405 | ENA

Lederbergia galactosidilytica strain:DSM 15595 | isolate:DSM 15595

Project description:Bacillus galactosidilyticus DSM 15595 genome sequencing

| PRJNA709590 | ENA