Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Identification of MELK-regulated genes in prostate cancer cells using next-generation sequencing

PROVIDER: PRJNA371482 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5232110.fastq.gz	Fastqsanger.gz
	SRR5232120.fastq.gz	Fastqsanger.gz
	SRR5232130.fastq.gz	Fastqsanger.gz
	SRR5232140.fastq.gz	Fastqsanger.gz
	SRR5232150.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 84

Similar Datasets

Identification of MELK-regulated genes in prostate cancer cells using next-generation sequencing

Project description:A cross-species analysis identified MELK as a potential therapeutic target in prostate cancer. To further elucidate the functional role of MELK in prostate cancer cells, we aimed to identify MELK-regulated genes. C4-2b cells were either treated with a small-molecule MELK inhibitor (OTSSP167), or transfected with siRNAs targeting MELK. Differentially expressed genes were identified using next-generation sequencing. Our results demonstrate that MELK promotes the expression of genes associated with tumour progression in prostate cancer cells.

2018-02-05 | GSE94570 | GEO

Identification of Hepatic Genes Regulated by ATF3 using Next Generation Sequencing

Project description:Identification of Hepatic Genes Regulated by ATF3 using Next Generation Sequencing

| PRJNA623854 | ENA

MELK and UBE2C gene knockdown in prostate cancer cell line PC3

Project description:In order to address the putative role of MELK and UBE2C in prostate cancer development and progression, we performed functional analysis upon siRNA-based knockdown, and searched for downstream genes and processes by microarray experiments. RNAi-based inhibition of MELK and UBE2C was efficient in PC3 prostate cancer cells and decreased transcriptional level down to about 30% remaining expression level.

2012-10-19 | GSE32873 | GEO

A Cross-Species Approach Identifies MELK as a Potential Therapeutic Target in Prostate Cancer

Project description:Genetically engineered mouse models of cancer represent valuable biological tools that can be used to filter genome-wide expression datasets generated from human prostate tumours, and identify gene expression alterations that are functionally important to cancer development and progression. In this study, we have generated RNASeq data from tumours arising in two established mouse models of prostate cancer, PB-Cre/PtenloxP/loxP and p53loxP/loxPRbloxP/loxP, and integrated this with published human prostate cancer expression data to pinpoint cancer-associated gene expression changes that are conserved between the two species. In order to identify potential therapeutic targets, we then filtered this information for genes that are either known or predicted to be druggable. Using this approach, we identified the serine/threonine kinase MELK as a potential therapeutic target in prostate cancer. MELK was overexpressed in both human and murine prostate cancers, and high expression of MELK was associated with biochemical recurrence in prostate cancer patients.

2018-02-05 | GSE94574 | GEO

Identification of stromally expressed molecules in the prostate by Tag profiling of cancer-associated fibroblasts, normal fibroblasts and fetal prostate

Project description:The stromal microenvironment plays key roles in prostate development and cancer. Cancer associated fibroblasts (CAFs) and other stromal cells stimulate tumourigenesis via several mechanisms including the expression of pro-tumourigenic factors. Mesenchyme (embryonic stroma) controls prostate organogenesis, and in some circumstances can re-differentiate prostate tumours. Epithelia are regulated by powerful paracrine signalling from the stroma in both development and disease, and identification of these stromal signals is important. We have applied next-generation Tag profiling to fetal human prostate, normal human prostate fibroblasts (NPFs) and CAFs to identify molecules expressed in prostatic stroma

2011-10-22 | GSE25018 | GEO

Identification of stromally expressed molecules in the prostate by Tag profiling of cancer-associated fibroblasts, normal fibroblasts and fetal prostate

2011-10-22 | E-GEOD-25018 | biostudies-arrayexpress

MELK and UBE2C gene knockdown in prostate cancer cell line PC3

2012-10-19 | E-GEOD-32873 | biostudies-arrayexpress

Identification of NEDD9 regulated genes in VCaP prostate cancer cells

Project description:Identification of NEDD9 regulated genes in VCaP prostate cancer cells

| PRJNA691065 | ENA

The role of MELK in melanoma progression

Project description:Survival kinase MELK has been shown to be important for proliferation of several tumors such as brain, breast or prostate. MELK expression is elevated in cutaneous melanoma, therefore, we analyzed the role of MELK in melanoma. Inhibition of MELK in melanoma cell lines resulted in decreased proliferation, increased cell death and decreased invasion. MELK expression is regulated through MAP kinase pathway and inhibition of MAPK pathway leads to diminished MELK expression. Finally, inhibition of MELK reduces tumor size in nude mice.

2020-12-30 | GSE83907 | GEO

Androgen Receptor-regulated genes in prostate cancer cells

Project description:Androgen Receptor-regulated genes in prostate cancer cells

| PRJNA434753 | ENA