Project description:We sought to determine the transcriptomic impacts of artemisinin, Artemisia annua extract, and Artemisia afra extract on M. tuberculosis. Log phase cultures were treated with lethal doses for four hours or with inhibitory or sub-inhibitory doses for 24 hours. RNA was collected from untreated controls at the same timepoint.
Project description:There is growing interest to apply proteomics to other organisms than just the biomedically relevant and important species such as human, mouse or rat. However, one of the main hurdles for successful application of proteomics to the organism of interest is still the availability of a well annotated and curated (genomic) database that can be used to search the (mainly MS-based) proteomic data for protein identification. Thus, the field of proteogenomics is increasingly becoming important with the aim to support the annotation of genomic sequence data by exploiting the information that is obtained through proteomics from the identification and characterization of the actual gene products/expression. Here, we have studied the organism Artemisia annua, which is a Chinese medicinal plant endemic to northern parts of China. A. annua is crucial to world health programs as it is currently the sole source for biosynthetically produced artemisinin, the anitmalarial pro-drug compound that has been the last line of defence against malaria for decades.
Project description:Artemisia annua is known to produce the antimalarial phytomolecule artemisinin. The seedling and mature leaf of the plant represent two contrasting tissues in terms of their artemisinin content. The major objective of the present study was to use a small-scale (750 target genes) microarray of A. annua for identification of genes that are differentially expressed in the seedling and mature leaf tissues of the plant.
