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Lipomyces starkeyi strain:NRRL Y11557 | isolate:NRRL Y11557

ABSTRACT: Lipomyces starkeyi NRRL Y11557 transcriptome

PROVIDER: PRJNA372984 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5487444_1.fastq.gz	Fastqsanger.gz
	SRR5487444_2.fastq.gz	Fastqsanger.gz
	SRR5487445_1.fastq.gz	Fastqsanger.gz
	SRR5487445_2.fastq.gz	Fastqsanger.gz

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Lipomyces starkeyi NRRL Y-11557

Project description:Lipomyces starkeyi NRRL Y-11557 Genome Sequencing Project

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Project description:Lipomyces genome scale model based on the Lipomyces starkeyi NRRL-11557 genome. Published in: Genome-Scale Model Development and Genomic Sequencing of the Oleaginous Clade Lipomyces Frontiers in Bioengineering and Biotechnology Industrial Biotechnology Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1356551

2024-03-20 | MODEL2403190001 | BioModels

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Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production.

Project description:BACKGROUND:Lipids from oleaginous yeasts emerged as a sustainable alternative to vegetable oils and animal fat to produce biodiesel, the biodegradable and environmentally friendly counterpart of petro-diesel fuel. To develop economically viable microbial processes, the use of residual feedstocks as growth and production substrates is required. RESULTS:In this work we investigated sugar beet pulp (SBP) and molasses, the main residues of sugar beet processing, as sustainable substrates for the growth and lipid accumulation by the oleaginous yeast Lipomyces starkeyi. We observed that in hydrolysed SBP the yeast cultures reached a limited biomass, cellular lipid content, lipid production and yield (2.5 g/L, 19.2%, 0.5 g/L and 0.08 g/g, respectively). To increase the initial sugar availability, cells were grown in SBP blended with molasses. Under batch cultivation, the cellular lipid content was more than doubled (47.2%) in the presence of 6% molasses. Under pulsed-feeding cultivation, final biomass, cellular lipid content, lipid production and lipid yield were further improved, reaching respectively 20.5 g/L, 49.2%, 9.7 g/L and 0.178 g/g. Finally, we observed that SBP can be used instead of ammonium sulphate to fulfil yeasts nitrogen requirement in molasses-based media for microbial oil production. CONCLUSIONS:This study demonstrates for the first time that SBP and molasses can be blended to create a feedstock for the sustainable production of lipids by L. starkeyi. The data obtained pave the way to further improve lipid production by designing a fed-batch process in bioreactor.

| S-EPMC7653891 | biostudies-literature

Expression analysis of Spathaspora passalidarum NRRL Y-27907 grown in glucose or xylose

Project description:Investigation of whole genome gene expression level changes in Spathaspora passalidarum NRRL Y-27907 grown aerobically in xylose, compared to the same strain grown aerobically in glucose. A six array study using total RNA recovered from three separate cultures of Spathaspora passalidarum NRRL Y-27907 grown in glucose and three separate cultures of Spathaspora passalidarum NRRL Y-27907 grown in xylose. Each array measures the expression level of 362,487 probes (average probe length 54.5 +/- 4.0 nt) tiled across the Spathaspora passalidarum NRRL Y-27907 genome with a median spacing distance of 29 nt. During data processing, probes are filtered to include only those probes corresponding to annotated protein-coding genes.

2011-10-19 | E-GEOD-24853 | biostudies-arrayexpress

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