Project description:Pathways underlying miRNA biogenesis, degradation, and activity were established early in land plant evolution, but the 24-nt siRNA pathway that guides DNA methylation was incomplete in early land plants, especially lycophytes. We show that the functional diversification of key gene families such as DICER-LIKE and ARGONAUTE (AGO) as observed in angiosperms occurred early in land plants followed by parallel expansion of the AGO family in ferns and angiosperms. We uncovered an unexpected AGO family specific to lycophytes and ferns. Our phylogenetic analyses of miRNAs in lycophytes, bryophytes, ferns, and angiosperms refined the temporal origination of conserved miRNA families in land plants.
Project description:Raw RNAseq data from ferns sampled in Singapore. Organ specific samples were selected for assembly via denovo assembly for comparative transcriptomics.
Project description:Bryophytes comprise mosses, liverworts and hornworts. The chromatin landscapes of mosses and liverworts are different, leaving open the question regarding the identity of the chromatin landscape of all bryophytes. To address this question we obtained a genome wide profile of 5-methylated cytosine from a model hornwort, Anthoceros agrestis.
Project description:This experiment probed for the presence of known Arabidopsis and rice microRNAs in total RNA samples derived from species representative of the major groups of land plants: Eudicots (Arabidopsis thaliana, Nicotiana benthamiana), monocots (Oryza satica, Triticum aestivum), magnoliids (Liriodendron tulipifera), gymnosperms (Pinus resinosa), ferns (Ceratopteris thalictroides), lycopods (Selaginella uncinata), and mosses (Polytrichum juniperinum). In most cases two technical or biological replicates were performed.
2005-05-01 | E-MEXP-293 | biostudies-arrayexpress
Project description:Transcriptomes of aquatic ferns
