Project description:Pathways underlying miRNA biogenesis, degradation, and activity were established early in land plant evolution, but the 24-nt siRNA pathway that guides DNA methylation was incomplete in early land plants, especially lycophytes. We show that the functional diversification of key gene families such as DICER-LIKE and ARGONAUTE (AGO) as observed in angiosperms occurred early in land plants followed by parallel expansion of the AGO family in ferns and angiosperms. We uncovered an unexpected AGO family specific to lycophytes and ferns. Our phylogenetic analyses of miRNAs in lycophytes, bryophytes, ferns, and angiosperms refined the temporal origination of conserved miRNA families in land plants.
2017-07-20 | GSE98408 | GEO
Project description:Transcriptomes of aquatic ferns
| PRJNA276721 | ENA
Project description:Comparative transcriptomes of Polychilos orchids
Project description:Raw RNAseq data from ferns sampled in Singapore. Organ specific samples were selected for assembly via denovo assembly for comparative transcriptomics.
Project description:To explore the mechanisms governing the leaf color variations in Chinese orchids, we analyzed gene expression differences between a wild-type and an albino-type cultivar of Cymbidium longibracteatum
Project description:The study of orchid mycorrhizal interactions is particularly complex because of the peculiar life cycle of these plants and their diverse trophic strategies. Here, large-scale transcriptomics has been applied to investigate gene expression in the mycorrhizal roots of the terrestrial mixotrophic orchid Limodorum abortivum under natural conditions. Our results provide new insights into the mechanisms underlying plant-fungus interactions in orchids and in particular on the plant responses to the mycorrhizal symbiont(s) in adult roots. Comparison with gene expression in mycorrhizal roots of another orchid species, Oeceoclades maculata, suggests that amino acids may represent the main nitrogen source in both protocorms and adult orchids, at least for mixotrophic species. The upregulation, in mycorrhizal L. abortivum roots, of some symbiotic molecular marker genes identified in mycorrhizal roots from other orchids as well as in arbuscular mycorrhiza, suggests a common plant core of genes in endomycorrhizal symbioses. Further efforts will be required to understand whether the specificities of orchid mycorrhiza depend on fine-tuned regulation of these common components, or whether specific additional genes are involved.
Project description:This experiment probed for the presence of known Arabidopsis and rice microRNAs in total RNA samples derived from species representative of the major groups of land plants: Eudicots (Arabidopsis thaliana, Nicotiana benthamiana), monocots (Oryza satica, Triticum aestivum), magnoliids (Liriodendron tulipifera), gymnosperms (Pinus resinosa), ferns (Ceratopteris thalictroides), lycopods (Selaginella uncinata), and mosses (Polytrichum juniperinum). In most cases two technical or biological replicates were performed.
