Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mycobacterium marinum

ABSTRACT: WhiB6 regulation of ESX-1 gene expression is controlled by a negative feedback loop in Mycobacterium marinum

PROVIDER: PRJNA389110 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5643030.fastq.gz	Fastqsanger.gz
	SRR5643031.fastq.gz	Fastqsanger.gz
	SRR5643032.fastq.gz	Fastqsanger.gz
	SRR5643023.fastq.gz	Fastqsanger.gz
	SRR5643024.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

The role of ESX-1 on infected and uninfected neutrophils in Mycobacterium marinum infection

Project description:Mice were infected with wild type or ESX-1-deficient (deltaRD1) Mycobacterium marinum. 14 days post infection, infected and uninfected neutrophils from the infected tissue were sorted for single cell RNA sequencing analysis.

2023-03-24 | GSE172072 | GEO

RNA sequencing data of Mycobacterium marinum treated with compound 8

Project description:The mechanisms of action of compound 8 in Mycobacterium marinum although it inhibits the secretion of the bacteria. In this experiment, we aim to investigated the effect of compound 8 on the global gene expression of Mycobacterium marinum

2023-02-14 | GSE224924 | GEO

miR-126 knockdown and Mycobacterium marinum infection

Project description:We identify a putative link between miR-126 knockdown and neuroactive ligand and notch signalling pathways in uninfected and Mycobacterium marinum infected zebrafish embryos.

2024-01-23 | GSE253537 | GEO

The loss of the PDIM/PGL virulence lipids causes differential secretion of ESX-1 sub-strates in Mycobacterium marinum

Project description:Raw and processed files in support of manuscript The loss of the PDIM/PGL virulence lipids causes differential secretion of ESX-1 sub-strates in Mycobacterium marinum The Virulence Lipid PDIM/PGL is essential for optimal protein secretion in Mycobacte-rium marinum WT, PDIM and complement strains lysate and culture filtrate analysis of proteomes

2023-12-21 | MSV000093713 | MassIVE

Temporal genome-wide fitness analysis of Mycobacterium marinum during infection reveals genetic requirement for virulence and survival in amoebae and microglial cells

Project description:Tuberculosis remains the most pervasive infectious disease and the recent emergence of multiple or even fully drug-resistant strains increases the risk and emphasizes the need for more efficient and better drug treatments. A key feature of mycobacteria pathogenesis, conserved between the human pathogen Mycobacterium tuberculosis and the model pathogen Mycobacterium marinum, is the metabolic switch to lipid catabolism during infection and altered expression of virulence genes in coordination with different stages of infection. This study aims at identifying genes that are involved in the establishment and maintenance of the infection. To achieve this, we have applied Transposon Sequencing (Tn-Seq) to M. marinum, an unbiased genome-wide strategy that combines saturation insertional mutagenesis and high throughput sequencing. This approach allowed us to precisely identify the localization and relative abundance of insertions in pools of transposon mutants. The essentiality of genes and the positive and negative fitness cost of mutations were quantitatively compared between in vitro growth and different stages of infection in two evolutionary distinct host phagocytes, the amoeba Dictyostelium discoideum and the murine BV2 microglial cells. We found that 57% of TA sites in the M. marinum genome were disrupted and that 568 genes (10.2%) were essential for M. marinum, which is comparable to previous Tn-Seq studies on M. tuberculosis and M. bovis. Major pathways involved in the survival of M. marinum during infection of D. discoideum were related to DNA damage repair, vitamin metabolism, the type VII secretion system (T7SS) ESX-1 and the Mce1 lipid transport system.

2023-12-01 | GSE249096 | GEO

Zebrafish response to Mycobacterium marinum infection (Affymetrix series)

Project description:Mycobacteria infect macrophages that aggregate with additional macrophages and lymphocytes to form granulomas. We have used a functional genomics approach to identify immune response genes expressed during granuloma formation in Mycobacterium marinum-infected transparent zebrafish larvae where individual infection steps can be viewed in real time. We assessed RNA expression profiles from zebrafish larvae that were either infected with Mycobacterium marinum, mock-infected, or uninfected. Zebrafish infections were performed at 1 day post-fertilization (dpf), and samples were derived from pools of 6dpf zebrafish larvae. Keywords: host response to infection

2008-01-01 | GSE8327 | GEO

Zebrafish response to Mycobacterium marinum infection (Compugen series)

Project description:Mycobacteria infect macrophages that aggregate with additional macrophages and lymphocytes to form granulomas. We have used a functional genomics approach to identify immune response genes expressed during granuloma formation in Mycobacterium marinum-infected transparent zebrafish larvae where individual infection steps can be viewed in real time. We assessed RNA expression profiles from zebrafish larvae that were either infected with Mycobacterium marinum or mock-infected. Zebrafish infections were performed at 1 day post-fertilization (dpf), and samples were derived from pools of 6dpf zebrafish larvae. Keywords: host response to infection

2008-01-01 | GSE8846 | GEO

Characterization of the immune response to Mycobacterium marinum infection in zebrafish

Project description:Mycobacterium marinum infection in zebrafish (Danio rerio) has been widely used to study human tuberculosis because the bacteria causing these two diseases are close relatives. We studied the zebrafish immune response to M. marinum infection through a whole-genome level transcriptome analysis. As expected based on the literature, our results showed the induction of genes coding proteins associated to immune signaling, cell migration and acute phase response indicating that the immune response to M. marinum infection in zebrafish is similar than the response to tuberculosis causing Mycobacterium tuberculosis in humans.

2019-10-16 | GSE118288 | GEO

Comparative transcriptomics of Mycobacterium bovis and Mycobacterium marinum

Project description:Related surrogate species are often used to study the molecular basis of pathogenicity of a pathogen on the basis of a shared set of biological features generally attributable to a shared core genome consisting of orthologous genes. An important and understudied aspect, however, is the extent to which regulatory features affecting the expression of such shared genes are present in both species. Here we report on an analysis of whole transcriptome maps for an important member of the TB complex Mycobacterium bovis and a closely related model organism for studying mycobacterial pathogenicity Mycobacterium marinum. Predict transcription start site

2014-09-08 | E-GEOD-51881 | biostudies-arrayexpress

Comparative transcriptomics of Mycobacterium bovis and Mycobacterium marinum

2014-09-08 | GSE51881 | GEO