Project description:We have utilized the raw sequence data from our earlier investigation of the lichen transcriptome to design a custom DNA microarray for C. rangiferina in order to study the transcripts expressed in lichen thallus during dehydration and rehydration. The aim of this study was to identify the genes most differentially expressed during the rehydration and drying processes and also to get a more integrative view of the molecular players who may play roles in the processes required for lichen desiccation tolerance and the rapid re-establishment of photosynthesis through functional annotation. 8 samples with three biological replicates for each sample, altogether 24 samples. D1h samples have been drying for 1 hour, D3h samples for 3 hours, D6h samples for 6 hours and Dry samples for 24 hours. W15m samples have been wetted for 15 minutes, W30m samples for 30 minutes, W1h samples for 1 hour and Wet samples for 3 hours.

2013-11-25 | E-GEOD-47624 | biostudies-arrayexpress

MAGE-seq E. coli primary sigma factor RpoD amplicon high-throughput genotyping

Project description:Genotyping of RpoD mutants via amplicon sequencing from the following manuscript: \\"Systematic dissection of σ70 sequence diversity and function in bacteria\\" by Park and Wang (2020). Includes raw sequencing reads from samples from MAGE-seq single codon saturation mutagenesis and high-throughput fitness competition experiment as well as the RpoD ortholog mutants generated through recombineering and CRISPR selection.

2022-06-06 | E-MTAB-9103 | biostudies-arrayexpress

Symbiodinium

Project description:Symbiodinium ITS2-Types Raw sequence reads

| PRJNA431693 | ENA