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Apostichopus japonicus

ABSTRACT: Differential expression of genes in the respiratory tree of sea cucumber (Apostichopus japonicus) under normal dissolved oxygen and hypoxia conditions

PROVIDER: PRJNA390546 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5683880.fastq.gz	Fastqsanger.gz
	SRR5683881.fastq.gz	Fastqsanger.gz
	SRR5683882.fastq.gz	Fastqsanger.gz
	SRR5683874.fastq.gz	Fastqsanger.gz
	SRR5683875.fastq.gz	Fastqsanger.gz

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Differential expression of genes in the respiratory tree of sea cucumber (Apostichopus japonicus) under normal dissolved oxygen and hypoxia conditions

Project description:The sea cucumber Apostichopus japonicus is a deposit-feeder and vital for marine benthic ecosystems. Hypoxia lasting for several days can lead to massive mortality in A. japonicus. It is important to understand the molecular responses of A. japonicus when exposed to acute changes in DO concentration. In the present study, RNA-seq provided a general overview of the gene expression profiles of the respiratory tree of A. japonicus exposed to dissolved oxygen (DO) of 8 mg/L (DO8), 4 mg/L (DO4) and 2 mg/L (DO2) conditions.

2018-12-31 | GSE100042 | GEO

Transcriptome landscape during tenderization in sea cucumber Apostichopus japonicus

Project description:The gene expression profiles associated with tenderization in body wall of sea cucumber (Apostichopus japonicus) were determined

2019-03-27 | GSE123077 | GEO

Differential gene expression in the intestine of sea cucumber (Apostichopus japonicus) under low and high salinity conditions

Project description:Sea cucumber, Apostichopus japonicus is a very important species for aquaculture, and its behavior and physiology can respond to the initial change in salinity. It is important to understand the molecular responses of A. japonicus when exposed to ambient changes in salinity In this study, RNA-seq provided a general overview of the gene expression profiles of the intestine of A. japonicus exposed to high salinity (SD40), normal salinity (SD30) and low salinity (SD20) environment.

2017-11-16 | GSE103466 | GEO

Lysine acetylproteome profiling reveals key acetylated proteins involved in heat shock response in the sea cucumber Apostichopus japonicus

Project description:Intestine of a total of 9 idividuals was sampled. The individuals were collected from 3 groups: Control (C), Heat stress 6h (HS6h) and Heat stress 48h (HS48h).After protein extraction,trypsin digestion and enrichment of Lys-acetylated peptides, the eluted peptides was applied with LC-MS/MS detection and data analysis.Lysine acetylproteome profiling reveals key acetylated proteins involved in heat shock response in the sea cucumber Apostichopus japonicus

2019-11-12 | PXD013929 | Pride

Apostichopus japonicus

Project description:High-Throughput Sequencing Reveals Differential Expression of miRNAs in respiratory tree from the sea cucumber Apostichopus japonicus Under Hypoxia Stress

| PRJNA392279 | ENA

Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation (part 2)

Project description:The sea cucumber Apostichopus japonicus withstands high water temperatures in the summer by suppressing metabolic rate and entering a state of aestivation. We hypothesized that changes in the expression of miRNAs could provide important post-transcriptional regulation of gene expression during hypometabolism via control over mRNA translation. The present study analyzed profiles of miRNA expression in the sea cucumber respiratory tree using Solexa deep sequencing technology. We identified 279 sea cucumber miRNAs, including 15 novel miRNAs specific to sea cucumber. Animals sampled during deep aestivation (DA; after at least 15 days of continuous torpor) were compared with animals from a non-aestivation (NA) state (animals that had passed through aestivation and returned to an active state). We identified 30 differentially expressed miRNAs ([RPM (reads per million) >10, |FC| (|fold change|) ≥1, FDR (false discovery rate) <0.01]) during aestivation, which were validated by two other miRNA profiling methods: miRNA microarray and real-time PCR. Among the most prominent miRNA species, miR-124, miR-124-3p, miR-79, miR-9 and miR-2010 were significantly over-expressed during deep aestivation compared with non-aestivation animals, suggesting that these miRNAs may play important roles in metabolic rate suppression during aestivation. analyze the global profile of small RNAs in non-aestivation (NA) and deep aestivation (DA) sea cucumbers' respiratory tree using Solexa sequencing technology

2014-10-01 | E-GEOD-52225 | biostudies-arrayexpress

Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus under heat stress

Project description:The high-throughput RNA-seq was applied under both 26°C and 30°C heat stress in Apostichopus japonicus, which revealed characterization of gene expression at sub-lethal and lethal limit temperature. The results will help to better understand the characteristics of heat shock response in A. japonicus.

2024-01-01 | GSE205975 | GEO

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Project description:Apostichopus japonicus

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Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation (part 2)

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Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation (part 1)

2014-10-01 | GSE52224 | GEO