Project description:To explore the mechanisms governing the leaf color variations in Chinese orchids, we analyzed gene expression differences between a wild-type and an albino-type cultivar of Cymbidium longibracteatum
Project description:Chinese orchid is a symbol of elegance and purity in China. Among the Chinese orchids, the leaf color variation of Cymbidium sinense (C. sinense) is the most abundant. The variation of leaf color makes the C. sinense more diversified and more valuable, however, its formation mechanism still needs more extensive exploration. Using TMT and LC-MS/MS analysis, the proteome and phos-phoproteome of C. sinense leaf variegation mutant were studied.
Project description:Cymbidium longibracteatum is a common cultivated species in the genus Cymbidium due to its elegant appearance, rich flower colors and strong fragrance, but its classification is quite controversial. In this study, the complete chloroplast genome of C. longibracteatum was obtained by Illumina sequencing. The chloroplast genome of C. longibracteatum is 150,070 bp in length with an overall GC content of 37.12%, which contains a large single-copy (LSC;84,949 bp) region, a small single-copy (SSC;13,745bp) region, and a pair of inverted repeats (IRs; 25,688 bp) regions. The genome contains 130 genes, namely 84 protein-coding genes, 38 tRNA genes and 8 rRNA genes. The maximum-likelihood phylogenetic tree has proved that C. longibracteatum should exist as an independent species in the genus Cymbidium, and it is most closely related to C. tortisepalum. This study provides valuable sequence resources for further study of C. longibracteatum.
