Ontology highlight
ABSTRACT:
PROVIDER: PRJNA391361 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5738150.fastq.gz | Fastqsanger.gz | |||
| SRR5738151.fastq.gz | Fastqsanger.gz | |||
| SRR5738152.fastq.gz | Fastqsanger.gz | |||
| SRR5738153.fastq.gz | Fastqsanger.gz | |||
| SRR5738154.fastq.gz | Fastqsanger.gz |
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Project description:We performed ATAC-seq on placenta dissected from e9.5 timed-pregnant mice in order to generate genome-wide chromatin accessibility maps. Integrating this data with previously published RNA-seq data showed that: 1) genes that have high expression and high ATAC-seq read coverage at the transcription start site (TSS) were enriched for house-keeping functions; 2) genes that have high expression and medium-low coverage at the TSS were enriched for placenta-specific genes and functions; 3) genes that have low expression and high TSS coverage are actively repressed. From the actively repressed genes, we extracted a protein-protein interaction network enriched for neuronal functions. These data increase our understanding of active and repressed gene networks in the e9.5 placenta. The analysis also provides a method for identifying repressed networks and tissue-specific genes by integrating ATAC-seq and RNA-seq data. Please note that the RNA-Seq data are already deposited and available under GSE65808.
2019-02-28 | GSE120599 | GEO
Project description:Single nuclei RNA-sequencing of the mouse placenta at four developmental stages (E9.5, E10.5, E12.5, E14.5). Supplementary files include the expression matrices for the integrated datasets containing nuclei from E9.5, E10.5, E12.5, and E14.5 and the clustering annotations for either all nuclei (AllStages_AllNuclei) or subclustered trophoblast nuclei (AllStages_TrophoblastNuclei). Processed data as R objects for both AllStages_AllNuclei and AllStages_TrophoblastNuclei are available at https://figshare.com/projects/Single_nuclei_RNA-seq_of_mouse_placental_labyrinth_development/92354
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Project description:Expression profiling of Prdm1 mutant E9.5 placenta was performed using Illumina whole genome V2 arrays. The hypothesis tested in the present study was that Blimp1 regulates the transcription of key genes involved in trophoblast differentiation. We demonstrate that the invading SpA-TGCs display robust Blimp1 expression and Blimp1 functional loss selectively disrupts specification of this discrete TGC sub-type. Transcriptional profiling experiments identified additional SpA-TGC lineage restricted marker genes that potentially regulate placental morphogenesis. Prdm1BEH/+ (Vincent et al., 2005) animals were intercrossed to generate null placental tissue. Total RNA obtained from 10 Prdm1+/+ and 11 Prdm1-/- E9.5 placenta samples was hybridized to Illumina WG6_V2 beadchips.
2012-07-23 | E-GEOD-39584 | biostudies-arrayexpress
Project description:The placenta is a transient organ that is repsonsible for multiple processes during pregnancy. Here we aim to study the development of the placenta using mouse models at embryonic day (e) e7.5, e8.5 and e9.5 by integrating RNA-seq data at the three timepoints to identify important genes, inferring their interaction networks, and predict novel regulators of placental development.
2022-11-21 | GSE202243 | GEO
Project description:TMT based quantification of protein abundance and phosphorylation state for developing mouse placenta. Timed-pregnant CD-1 mice were obtained from Charles Rivers Labs and dissected at e7.5 to extract the ectoplacental cones (EPCs) and at e9.5 to obtain the placenta as described by Martin and Cockroft (Martin, P.; Cockroft, D. L. Culture of Postimplantation Mouse Embryos). Staging of the mouse embryos was done according to Theiler criteria (Theiler, K. The House Mouse: Development and Normal Stages from Fertilization to 4 Weeks of Age; Springer-Verlag, 1972.)
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Project description:RNAseq analysis on e9.5 mutant mouse hearts
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Project description:Throughout pregnancy, the placenta is important for the well-being of the fetus, transporting nutrients and waste between the maternal and fetal blood supply, secreting hormones, and serving as a protective barrier. To obtain a more complete understanding of placental development, we must understand how placental gene expression is regulated. We used RNA-seq data and ChIP-seq data for the enhancer associated mark, H3k27Ac, to study gene regulation in the mouse placenta at embryonic day (e) 9.5, when the placenta is developing a complex network of blood vessels which facilitate maternal-fetal exchange of material. We identified several transcription factors upregulated at e9.5 with enriched binding sites in e9.5-specific enhancers. We performed follow up studies to investigate the role of the most enriched transcription factor, PLAGL1, in placental development. The PLAGL1 binding motif was found in 233 regions which were significantly associated with genes involved in vasculature development. To further understand the role of PLAGL1, we performed an siRNA knockdown in a human placental cell line and analyzed the genes that significantly changed in expression compared to cells treated with a negative control siRNA. Genes that significantly decreased in expression along with PLAGL1 were associated with placental vasculature development terms. Using a tube assay, we found that decreased PLAGL1 expression lead to reduced tube formation. By identifying PLAGL1 as an important regulator in the placenta, our analysis improves our understanding of gene regulation in the placenta, an important step towards developing methods to diagnose and treat placental diseases.
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Project description:Gastruloids are small aggregates of murine Embryonic Stem (ES) cells stimulated to undergo gastrulation-like symmetry breaking events in vitro (van den Brink, S. C. et al. 2014, Simunovic, M. & Brivanlou, A. H 2017). We analyzed the transcriptomic profiles of gastruloids at different time-points after aggregation (AA) and compared them with those of mouse embryo at different stages, raging from e6 to e9.5. We show that gastruloids are capable of organizing a post-occipital pattern of neural, mesodermal and endodermal derivatives that mimics that of the embryo in terms of gene expression.
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Project description:RNAseq from the following tissue samples: skeletal muscle; kidney; spleen; ovaries; placenta; castor gland; tail; toe webbing; whole blood; brain; lung; liver; heart; stomach; tongue; intestine
2019-07-02 | E-MTAB-8038 | biostudies-arrayexpress
Project description:The placenta is arguably the most anatomically variable organ in mammals. Understanding gene expression patterns among placentas from multiple species can illuminate the molecular basis of placenta function as well as potentially enrich our knowledge of the biology of obstetrical syndromes. We measured the expression profiles of the term placenta of multiple therian mammals using RNA-Seq. We identified multiple genes and transcripts which are differentially expressed across lineages including prolactin, relaxin, and galectins, as well as identifying the non-housekeeping genes which are core to placenta function (such as components of annexin complexes) in all species studied. RNAseq data from term placentas of 8 species with one sample per species
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