Project description:In this study, we used customized oligo-array in symbiotic association with the RNA-SEQ tool. Sequences generated previously in our lab through RNA-SEQ were validated on Agilent microarray platform. Gene expression in different tissues of Calotropis plant was studied. Whole plant was considered as control in this study and leaf, stem and root specific genes were mined out in reference to whole plant. Specific genes in a tissue, common genes in all the tissues as well as highly abundant genes were identified from the dataset.
Project description:To explore the wound response in C.procera seedlings, a customized microarray chip (8x60K) was developed using the annotated transcripts obtained from de novo transcriptome data of C.procera. 3-month-old seedlings were subjected to mechanical wounding for 1h, 3h and 6 hours and RNA was isolated for the microarray hybridization and analysis. Expression analysis of transcripts involved in defense, primary metabolism, secondary metabolism, TFs were studied in comparison of control seedlings.
