Pseudomonas aeruginosa PAO1 strain:PA01
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA400528 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:A novel Cyclic di-GMP modulator of PrtR controls bacterial virulence in Pseudomonas aeruginosa
| PRJNA401973 | ENA
Project description:Pseudomonas aeruginosa, a common inhabitant of soil and water, is an opportunistic pathogen of growing clinical relevance. Its genome, one of the largest among bacteria [5570 open reading frames (ORFs)] approaches that of simple eukaryotes. We have constructed a comprehensive gene collection for this organism utilizing the annotated genome of P. aeruginosa PA01 and a highly automated and laboratory information management system (LIMS)-supported production line. All the individual ORFs have been successfully PCR-amplified and cloned into a recombination-based cloning system. We have isolated and archived four independent isolates of each individual ORF. Full sequence analysis of the first isolate for one-third of the ORFs in the collection has been completed. We used two sets of genes from this repository for high-throughput expression and purification of recombinant proteins in different systems. The purified proteins have been used to set up biochemical and immunological assays directed towards characterization of histidine kinases and identification of bacterial proteins involved in the immune response of cystic fibrosis patients. This gene repository provides a powerful tool for proteome- and genome-scale research of this organism, and the strategies adopted to generate this repository serve as a model for building clone sets for other bacteria.
| S-EPMC528936 | biostudies-literature
Project description:The P. aeruginosa reference strain PAO1 has been used to delineate much of the physiology, metabolism, and fundamental biology of the species. The wild-type parent of PAO1 was lost, and PAO1 carries a regulatory mutation introduced for positive genetic selection that affects antibiotic resistance, virulence, quorum sensing, and other traits. The mutation is a loss-of-function change in an oxidoreductase gene (mexS), which constitutively activates a stress response controlled by a positive regulator (MexT). Fitness defects associated with the constitutive response have led to the inadvertent selection of mexT-minus suppressor mutations, creating genetic heterogeneity in PAO1 sublines studied in different laboratories. To help circumvent complications due to the mexS-minus phenotypes, we created a wild-type version of PAO1 (called LPAO) by "reverting" its mexS to the functional allele likely to have been in its parent. Phenotypic analysis revealed that the mexS-minus allele in PAO1 makes growth sensitive to salt (NaCl) and is lethal when combined with mutations inactivating the major sodium antiporter (ShaABCDEF). The salt sensitivity of PAO1 may underlie some complex mexS-minus phenotypes and help explain the selection of mexT-minus suppressor mutations. To facilitate genetic comparisons of PAO1, LPAO, and other P. aeruginosa strains, we developed a transformation procedure to transfer selectable alleles, such as transposon insertion alleles, between strains. Overall, the study helps explain phenotypic heterogeneity of PAO1-derived strains and provides resources to help recognize and eliminate difficulties due to it. IMPORTANCE The P. aeruginosa reference strain PAO1 carries a regulatory mutation that may affect processes characterized in it. To eliminate complications due to the mutation, we constructed a version of the missing wild-type parent strain and developed methods to transfer mutations between PAO1 and the new strain. The methods are likely to be applicable to other isolates of P. aeruginosa as well.
| S-EPMC8223932 | biostudies-literature
Project description:Application of 200 µA of direct current to bacterial biofilms leads to cellular death. We hypothesize that bacterial death is caused, in part, by the generation of reactive oxygen species. We used microarray analysis to determine the effects at a cellular level following 60 minutes of exposure of bacterial biofilms to 200 µA direct current.
2016-12-02 | GSE90757 | GEO
Project description:Pseudomonas aeruginosa PAO1 dispersed cells transcription as assayed by RNA-Seq
| PRJNA229796 | ENA
Project description:Pseudomonas aeruginosa PAO1, PA01-pYedQ, pelpsl-pYedQ transcription as assayed by RNA-Seq
| PRJNA229797 | ENA
Project description:Reducing intracellular c-di-GMP content increases quorum sensing-regulated virulence in Pseudomonas aeruginosa
| PRJNA381683 | ENA
Project description:Pseudomonas aeruginosa PAO1 transcription under Iberin treatment as assayed by RNA-Seq
| PRJNA213564 | ENA
Project description:Pseudomonas aeruginosa Transcriptome
| PRJNA264943 | ENA
Project description:Pseudomonas aeruginosa PAO1 dispersed cells project as assayed by RNA-Seq_Batch1_MiSeq
| PRJNA246577 | ENA