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Apostichopus japonicus

ABSTRACT: Differential gene expression in the intestine of sea cucumber (Apostichopus japonicus) under low and high salinity conditions

PROVIDER: PRJNA401773 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6004440.fastq.gz	Fastqsanger.gz
	SRR6004441.fastq.gz	Fastqsanger.gz
	SRR6004442.fastq.gz	Fastqsanger.gz
	SRR6004443.fastq.gz	Fastqsanger.gz
	SRR6004444.fastq.gz	Fastqsanger.gz

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Differential gene expression in the intestine of sea cucumber (Apostichopus japonicus) under low and high salinity conditions

Project description:Sea cucumber, Apostichopus japonicus is a very important species for aquaculture, and its behavior and physiology can respond to the initial change in salinity. It is important to understand the molecular responses of A. japonicus when exposed to ambient changes in salinity In this study, RNA-seq provided a general overview of the gene expression profiles of the intestine of A. japonicus exposed to high salinity (SD40), normal salinity (SD30) and low salinity (SD20) environment.

2017-11-16 | GSE103466 | GEO

Transcriptome landscape during tenderization in sea cucumber Apostichopus japonicus

Project description:The gene expression profiles associated with tenderization in body wall of sea cucumber (Apostichopus japonicus) were determined

2019-03-27 | GSE123077 | GEO

Lysine acetylproteome profiling reveals key acetylated proteins involved in heat shock response in the sea cucumber Apostichopus japonicus

Project description:Intestine of a total of 9 idividuals was sampled. The individuals were collected from 3 groups: Control (C), Heat stress 6h (HS6h) and Heat stress 48h (HS48h).After protein extraction,trypsin digestion and enrichment of Lys-acetylated peptides, the eluted peptides was applied with LC-MS/MS detection and data analysis.Lysine acetylproteome profiling reveals key acetylated proteins involved in heat shock response in the sea cucumber Apostichopus japonicus

2019-11-12 | PXD013929 | Pride

Differential expression of genes in the respiratory tree of sea cucumber (Apostichopus japonicus) under normal dissolved oxygen and hypoxia conditions

Project description:The sea cucumber Apostichopus japonicus is a deposit-feeder and vital for marine benthic ecosystems. Hypoxia lasting for several days can lead to massive mortality in A. japonicus. It is important to understand the molecular responses of A. japonicus when exposed to acute changes in DO concentration. In the present study, RNA-seq provided a general overview of the gene expression profiles of the respiratory tree of A. japonicus exposed to dissolved oxygen (DO) of 8 mg/L (DO8), 4 mg/L (DO4) and 2 mg/L (DO2) conditions.

2018-12-31 | GSE100042 | GEO

Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation (part 2)

Project description:The sea cucumber Apostichopus japonicus withstands high water temperatures in the summer by suppressing metabolic rate and entering a state of aestivation. We hypothesized that changes in the expression of miRNAs could provide important post-transcriptional regulation of gene expression during hypometabolism via control over mRNA translation. The present study analyzed profiles of miRNA expression in the sea cucumber respiratory tree using Solexa deep sequencing technology. We identified 279 sea cucumber miRNAs, including 15 novel miRNAs specific to sea cucumber. Animals sampled during deep aestivation (DA; after at least 15 days of continuous torpor) were compared with animals from a non-aestivation (NA) state (animals that had passed through aestivation and returned to an active state). We identified 30 differentially expressed miRNAs ([RPM (reads per million) >10, |FC| (|fold change|) ≥1, FDR (false discovery rate) <0.01]) during aestivation, which were validated by two other miRNA profiling methods: miRNA microarray and real-time PCR. Among the most prominent miRNA species, miR-124, miR-124-3p, miR-79, miR-9 and miR-2010 were significantly over-expressed during deep aestivation compared with non-aestivation animals, suggesting that these miRNAs may play important roles in metabolic rate suppression during aestivation.

2014-10-01 | GSE52225 | GEO

Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation (part 1)

2014-10-01 | GSE52224 | GEO

Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation (part 2)

2014-10-01 | E-GEOD-52225 | biostudies-arrayexpress

sea cucumber Apostichopus japonicus

Project description:sea cucumber Apostichopus japonicus Raw sequence reads

| PRJNA622877 | ENA

Apostichopus japonicus circumoral nerve ring LC-MS/MS

Project description:The objective of this study was to perform a detailed analysis of neuropeptides in A. japonicus by sequencing the transcriptome of neural tissue (circumoral nerve ring; CNR) and combining analysis of these sequence data with mass spectroscopic analysis of CNR extracts so that the structure of mature neuropeptides could be determined. Collectively, these data provide an important molecular basis for investigation of the physiological roles of neuropeptides in A. japonicus and other sea cucumber species.

2019-06-25 | PXD013818 | Pride

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Project description:High-throughput Sequencing Reveals Differential expression of MicroRNAs during intestine regeneration of sea cucumber Apostichopus japonicus

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