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Differential gene expression induced by anti-cancer agent plumbagin is mediated by androgen receptor in prostate cancer cells

ABSTRACT: Differential gene expression induced by anti-cancer agent plumbagin is mediated by androgen receptor in prostate cancer cells

PROVIDER: PRJNA412309 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6080080.fastq.gz	Fastqsanger.gz
	SRR6080090.fastq.gz	Fastqsanger.gz
	SRR6080081.fastq.gz	Fastqsanger.gz
	SRR6080091.fastq.gz	Fastqsanger.gz
	SRR6080082.fastq.gz	Fastqsanger.gz

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Similar Datasets

Differential gene expression induced by anti-cancer agent plumbagin is mediated by androgen receptor in prostate cancer cells

Project description:Plumbagin treatment of mice harboring PTEN-P2 tumors in the prostate or on prostate tissue maintained in vivo results in tumor regression when coupled with androgen deprivation therapy. This suggested that dihydrotestosterone (DHT) production in the testes prevents tumor regression due to plumbagin treatment by an unknown mechanism. We performed RNA-seq analysis on cells treated with plumbagin, DHT, or both, and analyzed differential gene expression using RNA-seq. DHT and plumbagin synergize to differentially regulate many genes that are not differentially regulated by either agent alone. For many genes, increases in mRNAs caused by DHT are sharply down-regulated by plumbagin, and some transcripts increase or decrease in response to plumbagin in a DHT-dependent manner. We also observed that plumbagin causes extensive RNA damage independently of DHT. We also include data from a simple time course experiment in which gene expression in response to DHT was studied using RNA-seq.

2017-09-28 | GSE104305 | GEO

Differential gene expression induced by anti-cancer agent plumbagin is mediated by androgen receptor in prostate cancer cells

Project description:Differential gene expression induced by anti-cancer agent plumbagin is mediated by androgen receptor in prostate cancer cells

| PRJNA412309 | ENA

Differential gene expression induced by anti-cancer agent plumbagin is mediated by androgen receptor in prostate cancer cells.

Project description:Treatment of mice harboring PTEN-P2 tumors in the prostate or on prostate tissue in vivo with 5-hydroxy-2-methyl-1,4-naphthoquinone, also known as plumbagin, results in tumor regression in castrated mice, but not in intact mice. This suggested that dihydrotestosterone (DHT) production in the testes may prevent cell death due to plumbagin treatment, but the underlying mechanism is not understood. We performed RNA-seq analysis on cells treated with combinations of plumbagin and DHT, and analyzed differential gene expression, to gain insight into the interactions between androgen and plumbgin. DHT and plumbagin synergize to alter the expression of many genes that are not differentially regulated by either single agent when used alone. These experiments revealed that, for many genes, increases in mRNAs caused by DHT are sharply down-regulated by plumbagin, and that many transcripts change in response to plumbagin in a DHT-dependent manner. This suggests that androgen receptor mediates some of the effects of plumbagin on gene expression.

| S-EPMC5807367 | biostudies-literature

Expression data from MCF-7 breast cancer cells treated with Plumbagin (PL) and its derivative Acetyl Plumbagin (AP)

Project description:The study aimed at finding the molecular mechanism of action of PL and AP in breast cancer cells. The dataset shows the comparison of gene expression in Plumbagin/Acetyl Plymbagin treated MCF-7 cells when compared with untreated samples.

2015-04-28 | GSE68026 | GEO

A small molecule BAG1 inhibitor as therapeutic agent for androgen receptor positive prostate cancer

Project description:A small molecule BAG1 inhibitor as therapeutic agent for androgen receptor positive prostate cancer

| PRJNA755637 | ENA

Expression data from plumbagin-treated B16F10

Project description:Gene expression profiling reveals a potential role of plumbagin on the induction of B16F10 metastasis cells were treated with 0 and 2 µM plumbagin for 24 h ; Microarray gene expression profiling was conducted.

2020-06-27 | GSE153324 | GEO

Mycobacterium tuberulosis H37Rv strains: Control vs. Plumbagin

2010-09-20 | GSE24045 | GEO

Differential gene expression profiles between two novel murine prostate cancer cell lines derived from androgen‑dependent (PLum‑AD cells) and androgen‑independent (PLum‑AI cells) prostate cancer

Project description:Orthotopic tumors were previously generated from parental Prostate Luminal (PLum) cells under androgen‑dependent (PLum-AD) and androgen‑independent (PLum-AI) conditions in order to establish cellular models of prostate cancer progression (Abou-Kheir et al., 2011; doi: 10.1371/journal.pone.0026112). We used microarrays to evaluate the differential gene expression profiles underlying progression of prostate cancer from primary androgen-dependent stage to advanced androgen-independent stage using newly isolated murine prostate cancer cell lines. Those cell lines represent novel in vitro models of androgen‑dependent and –independent prostate cancer, recapitulating the progression of the disease to a more invasive phenotype upon androgen deprivation.

2020-05-27 | GSE151187 | GEO

Changes in miRNA expression profile upon treatment with plumbagin or menadione in SW-620 colorectal cancer cells

Project description:To elucidate the mode of actions of Plumbagin and Menadione, miRNA-profilling was performed after treating cells with vehicle (DMSO), Plumbagin (10uM), and Menadione (10uM) for 24 hours. Threel replicates for control and Menadione and two replicates for Plumbagin were subjected to miRNA-microarray.

2023-03-01 | GSE197300 | GEO

Mycobacterium tuberulosis H37Rv strains: Control vs. Plumbagin

Project description:Transcriptional profiling of Mycobacterium tuberculosis H37Rv strains comparing control DMSO treated strains with Plumbagin treated strains. Goal was to determine the effects of Plumbagin against Mycobacterium tuberculosis H37Rv strains. Two-condition experiment,control DMSO vs. Plumbagin. Biological replicates: 2 control replicates, 2 Plumbagin replicates. The file showing raw and normalized data (~4645 rows) extracted from Agilent Feature Extraction Software is linked below. Samples details are described in PDF file linked below (NOTE: The samples 'Plumbagin' and 'chelerythrine' represent the submissions to GEO).

2010-09-20 | E-GEOD-24045 | biostudies-arrayexpress

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