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Transcriptomics study of three Blumeria graminis formae speciales

ABSTRACT: Transcriptomics study of three Blumeria graminis formae speciales

PROVIDER: PRJNA427159 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6410410.fastq.gz	Fastqsanger.gz
	SRR6410420.fastq.gz	Fastqsanger.gz
	SRR6410411.fastq.gz	Fastqsanger.gz
	SRR6410421.fastq.gz	Fastqsanger.gz
	SRR6410412.fastq.gz	Fastqsanger.gz

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Project description:With this experiment we aimed do identify eventual genes that are differentially expressed by the fungal pathogen Blumeria graminis triticale when it grows on two different hosts (wheat and triticale) We used to fungal isolates, for each of them we infected wheat and triticale and we extracted RNA (and sequenced) from the infected plant tissue. Three technical replicates for each combinations plant-pathogen were used

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Iso-Seq analysis of barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei; isolate 5874)

Project description:Purpose: The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012. Molecular Plant Pathology 13 (4): 414-430. DOI: 10.1111/j.1364-3703.2011.00783.x). Blumeria graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to discover novel transcripts expressed following barley infection with blumeria.

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Barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei; isolate 5874)

Project description:Purpose: The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012). B. graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to address the temporal regulation of membrane trafficking associated gene expression in barley-powdery mildew interactions. We created an isogenic panel of immune signaling mutants to address three main questions: (i) which Blumeria secreted proteins are differentially regulated in response to different compromised genotypes, (ii) which barley membrane trafficking genes are altered in response to pathogen attack, and (iii) how are these genes interacting across genotypes and infection stages.

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