Browse
Submit Data
Databases
API
Help

Dataset Information

493 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Blumeria graminis f.sp. hordei DH14 proteome 2nd analysis incl. CSEPs

ABSTRACT: Large scale proteomics of Blumeria graminis f.sp. hordei DH14 has been conducted with a new ORF database containing candidate secreted effector proteins (CSEPs). With a comparative approach, CSEPs only deteted in Haustoria (the interacting cells) containing tissue were identified.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Blumeria Graminis F. Sp. Hordei Dh14

TISSUE(S): Conidiophore, Hypha, Conidium, Haustorium

SUBMITTER: Laurence v. Bindschedler

PROVIDER: PRD000730 | Pride | 2012-12-06

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_26886.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_26886.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_26886.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_26887.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_26887.pride.mztab.gz	Mztab

Items per page:

1 - 5 of 12

Similar Datasets

LC-MSMS of Hordeum vulgare epidermis containing haustoria from Blumeria graminis f.sp. hordei

Project description:Blumeria graminis f.sp. hordei is an obligate biotrohic fungal pathogen causing powdery mildew in barley. As for other biotrophic fungi, haustorial structures are at the centre of the biotrophic interaction and molecular exchanges, delivering fungal effectors or virulence factors, and taking nutrient from the host. Haustoria are originiated by the fungus, following successful penetration of the initial penetration peg through the plant cell call. Haustorial structures mainly of fungal origin, but they are surrounding by a plant component, the extrauhaustorial membrane and matrix (EHM and EHMx) forming the extrahuastorial complex (EHMc). The plant protein make-up of the plant extrahaustorial components remained unexplored, and this is a first study trying to describe plant proteome associated with haustoria using samples enriched for these structures. Therefore, proteomes of haustoria enriched samples from the epidermis of barley leaves infected with Blumeria graminins f.sp. hordei, the causing agent of barley powdery mildew, were compared to infected epidermis and un-infected epidermis to identify haustoria associated plant proteins. Haustoria were enriched from infected epidermis by digesting epidermal cell walls with cell wall degrading enzymes prior to enrichment for haustorial structures. Proteins identified in these samples were compared to infected and uninfected epidermis samples using a non-targeted label free semi-quantitation method.

2019-11-01 | PXD012684 | Pride

Transcription profiling by high throughput of Blumeria gramis during infection of Barley

Project description:The aim of this experiment was to investigate the transcriptomic variation within Blumeria graminis f. sp. hordei during infection of Barley. SOLiD RNA-seq data was generated for 6 samples, three from the epiphytic hyphae and three from the haustoria within the epidermis.

2012-06-11 | E-MTAB-682 | biostudies-arrayexpress

Gene expression analysis of RACB-transgenic barley plant lines in interaction with Blumeria graminis f.sp. hordei

Project description:To analyze RACB- and Bgh (Blumeria graminis f.sp. hordei)-dependently expressed genes we used wild type and transgenic barley misexpressing the susceptibility factor RACB

2017-03-27 | GSE69215 | GEO

mRNA sequence data of three powdery mildew fungi - GcM3, OnM2 and GcC1 (mycelia and haustoria containing infected plant leaves)

Project description:Powdery mildew (PM) is one of the most important and widespread plant diseases caused by obligate biotrophic Ascomycete fungi in the order of Erysiphales. Monocot PM fungi such as Blumeria graminis f.sp. hordei (Bgh) infectious on barley and B. graminis f.sp. tritici (Bgt) infectious on wheat exhibit high-level of host-specialization. By contrast, many dicot PM fungi display rather broad host ranges. To understand why different PM fungi adopt distinct modes of host-adaption, we sequenced the genomes of four dicot PM strains belonging to Golovinomyces cichoracearum (GcC1, GcM1, GcM3) or Oidium neolycopersici (OnM2) and conducted comparative sequence analyses. PM fungi have highly repetitive genomes that are difficult to perform gene prediction. By combing RNA-seq expression evidence with ab initio gene prediction, we successfully improved the number of predicted genes from 4000 to 6000. By comparing the transcriptional profiling from haustoria with mycelia in OnM2 and GcM3, we found that 86%-96% of the predicted genes are expressed in mycelia and/or haustoria, indicating an efficient expression system of PM fungi. Besides, our results showed that gene regulation mechanisms in haustorial cells maybe under gone a much higher level of diversification between OnM2 and GcM3, since they share only a small proportion (21%) of genes up-regulated in huastoria cells. Notably, a higher proportion of candidate effector genes are selectively up-regulated in haustorial cells, agreeing with their function in suppressing host defense and facilitating nutrient uptake.

2018-06-01 | GSE85906 | GEO

Iso-Seq analysis of barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei; isolate 5874)

Project description:Purpose: The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012. Molecular Plant Pathology 13 (4): 414-430. DOI: 10.1111/j.1364-3703.2011.00783.x). Blumeria graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to discover novel transcripts expressed following barley infection with blumeria.

2021-03-30 | GSE165730 | GEO

Transcription profiling time series of leaves from Hordeum vulgare, Ingrid infected with Blumeria graminis f. sp. hordei or Blumeria graminis f. sp. tritici

Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid (leaf) and Blumeria graminis f. sp. tritici with Hordeum vulgare, Ingrid (leaf)

2009-06-10 | E-IPKG-7 | biostudies-arrayexpress

Transcription profiling time series of leaf epidermis from Hordeum vulgare Ingrid infected with Blumeria graminis f. sp. hordei and Blumeria graminis f. sp. tritici

Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid (leaf epidermis) and Blumeria graminis f. sp. tritici with Hordeum vulgare, Ingrid (leaf epidermis)

2009-06-10 | E-IPKG-8 | biostudies-arrayexpress

Transcription profiling time series of leaves from Hordeum vulgare, Ingrid BC7 mlo5 infected with Blumeria graminis f. sp. hordei

Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid BC7 mlo5 (leaf)

2009-04-01 | E-IPKG-5 | biostudies-arrayexpress

Transcription profiling time series of leaf epidermis from Hordeum vulgare Ingrid BC7 mlo5 infected with Blumeria graminis f. sp. hordei

Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid BC7 mlo5 (leaf epidermis)

2009-04-01 | E-IPKG-4 | biostudies-arrayexpress

Transcription profiling of barley Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13)

Project description:A large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes differentially expressed in incompatible (resistant) vs. compatible (susceptible) and Mla-specified Rar1-dependent vs. -independent interactions. A split-split-plot design with 108 experimental units (3 replications x 2 isolates x 3 genotypes x 6 time points) was used to profile near-isogenic lines containing the Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13).

2004-09-01 | E-MEXP-142 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data