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Blumeria graminis f.sp. hordei DH14 proteome 2nd analysis incl. CSEPs

ABSTRACT: Large scale proteomics of Blumeria graminis f.sp. hordei DH14 has been conducted with a new ORF database containing candidate secreted effector proteins (CSEPs). With a comparative approach, CSEPs only deteted in Haustoria (the interacting cells) containing tissue were identified.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Blumeria Graminis F. Sp. Hordei Dh14

TISSUE(S): Conidiophore, Hypha, Conidium, Haustorium

SUBMITTER: Laurence v. Bindschedler

PROVIDER: PRD000730 | Pride | 2012-12-06

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_26886.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_26886.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_26886.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_26887.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_26887.pride.mztab.gz	Mztab

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Project description:Blumeria graminis f.sp. hordei is an obligate biotrohic fungal pathogen causing powdery mildew in barley. As for other biotrophic fungi, haustorial structures are at the centre of the biotrophic interaction and molecular exchanges, delivering fungal effectors or virulence factors, and taking nutrient from the host. Haustoria are originiated by the fungus, following successful penetration of the initial penetration peg through the plant cell call. Haustorial structures mainly of fungal origin, but they are surrounding by a plant component, the extrauhaustorial membrane and matrix (EHM and EHMx) forming the extrahuastorial complex (EHMc). The plant protein make-up of the plant extrahaustorial components remained unexplored, and this is a first study trying to describe plant proteome associated with haustoria using samples enriched for these structures. Therefore, proteomes of haustoria enriched samples from the epidermis of barley leaves infected with Blumeria graminins f.sp. hordei, the causing agent of barley powdery mildew, were compared to infected epidermis and un-infected epidermis to identify haustoria associated plant proteins. Haustoria were enriched from infected epidermis by digesting epidermal cell walls with cell wall degrading enzymes prior to enrichment for haustorial structures. Proteins identified in these samples were compared to infected and uninfected epidermis samples using a non-targeted label free semi-quantitation method.

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