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Transcriptomic analysis of HCT116 cells cultured under different oxygen conditions

ABSTRACT: Transcriptomic analysis of HCT116 cells cultured under different oxygen conditions

PROVIDER: PRJNA430679 | ENA |

REPOSITORIES: ENA

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Transcriptomic analysis of HCT116 cells cultured under different oxygen conditions

Project description:To explore the molecular basis of hypoxic response, we analyzed gene expression profiles of HCT116 cells cultured under different oxygen conditions with or without inhibition of P53 signaling or fatty acid b oxidation (FAO).

2021-01-07 | GSE109318 | GEO

Replication maps of HCT116 cells grown under various conditions

Project description:Replication maps of HCT116 cells grown under various conditions

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Changes in gene expression of Imr90 cells with age and senescence under 3% and 20% oxygen growth conditions

Project description:Senescent is an irreversible form of cell cycle arrest initiated by damaged cell constituents and subsequent pro-oncogenic signaling. Replicative senescence in vitro can be considered a model for human aging. When fibroblasts are cultured under atmospheric oxygen conditions of 20%, typical of normal tissue culture procedure, fibroblasts generally reach their replicative capacity at 50-60 population doublings (PDs). When fibroblasts are cultured under normal physiological oxygen conditions of 3%, PDs increase about 30% relative to atmospheric levels. Hence while oxygen is a requirement for normal aerobic respiration, it can contribute to the total amount of oxidative stress to which cells are exposed to, leading to a long-term adverse effect in vitro. Inasmuch, cultures maintained under hyperoxic and hypoxic conditions provide a convenient model system for assessing the relationship between oxygen/oxidative stress and senescence. We used microarrays to profile the changes in global gene expression during aging and senescence of Imr90 cells under growth oxygen conditions of 3% and 20%.

2010-03-01 | GSE19018 | GEO

Rhizopus delemar grown under high and low oxygen conditions.

Project description:Rhizopus delemar grown under high and low oxygen conditions.

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Changes in gene expression of Imr90 cells with age and senescence under 3% and 20% oxygen growth conditions

2010-03-06 | E-GEOD-19018 | biostudies-arrayexpress

Memory CD8+ T cells upregulate glycolysis and effector functions under limiting oxygen conditions

Project description:Memory CD8+ T cells are indispensable for maintaining long-term immunity against intracellular pathogens and tumors. Despite their presence in oxygen-deprived tissues of infection sites or tumors, the impact of local oxygen pressure on memory CD8+ T cells has remained largely unclear. We sought to elucidate how oxygen pressure impacted memory CD8+ T cells arising after infection with Listeria monocytogenes-OVA. Our data revealed that reduced oxygen pressure during in vitro culture switched CD8+ T cell metabolism from an OXPHOS to a glycolytic phenotype. Quantitative proteomic analysis showed that limiting oxygen conditions increased the expression of glucose transporters and components of the glycolytic pathway, while decreasing TCA cycle and mitochondrial respiratory chain proteins. The altered CD8+ T cell metabolism did not affect the expansion potential, but enhanced the granzyme B and IFN- production capacity. Memory CD8+ T cells cultured under low oxygen pressure were able to persist long-term in vivo and provided protection against bacterial rechallenge. Taken together, our study indicates that strategies of cellular immune therapy may benefit from reducing oxygen during culture to develop memory CD8+ T cells with superior effector functions .

2022-12-01 | PXD032236 | Pride

mTORC inhibition of neuroblastoma SK-N-BE(2)c cells cultured under hypoxic conditions

Project description:Differential expression of the oxygen sensitive hypoxia-inducible transcription factor (HIF) subunits HIF-1a and HIF-2a occurs in many tumor types, but the underlying regulatory mechanisms remain poorly understood. Here we investigate the role of mTOR complex in the regulation of HIF expression in cells cultured under hypoxic conditions.

2016-01-01 | GSE69833 | GEO

Gene expression of murine long-lived bone marrow plasma cells ex vivo and plasma cells cultured for 3 days with ST2 stromal cells and the cytokine APRIL under physiological oxygen conditions.

Project description:We compared gene expression profiles of murine longlived bone marrow plasma cells isolated from immunized C57BL/6J in the memory phase with plasma cells co-cultured for 3 days with murine ST2 stromal cells in the presence of the cytokine APRIL under physiological oxygen conditions.

2019-12-01 | GSE107206 | GEO

Transcriptomic analysis of the oxygen response in Saccharomyces cerevisiae EC1118 under wine fermentation conditions

Project description:Oxygen additions play a critical role in winemaking. However, few studies have focused on how this oxygen affects yeast metabolism and physiology in wine making conditions. We performed microarrays to unveil the oxygen response in wine making conditions.

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Analysis of Thermosynechococcus elongatus BP1 and Meiothermus ruber strain A co-cultured under variable light and oxygen conditions

Project description:Analysis of Thermosynechococcus elongatus BP1 and Meiothermus ruber strain A co-cultured under variable light and oxygen conditions

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