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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [Custom mRNA array]

ABSTRACT: Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [Custom mRNA array]

PROVIDER: PRJNA434041 | ENA |

REPOSITORIES: ENA

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [Custom mRNA array]

Project description:Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAprotect. Gene expression analysis was performed against clone-, patient-, and passage-matched ground control samples.

2018-07-26 | GSE110560 | GEO

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

Project description:Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAProtect, total RNA purification, and microRNA expression analysis against clone-, patient-, and passage-matched ground control samples.

2018-07-26 | GSE110561 | GEO

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

2018-07-26 | GSE110562 | GEO

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

Project description:Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

| PRJNA433985 | ENA

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

Project description:Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

| PRJNA434040 | ENA

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

Project description:Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

| PRJNA434039 | ENA

Behavior of mice aboard the International Space Station.

Project description:Interest in space habitation has grown dramatically with planning underway for the first human transit to Mars. Despite a robust history of domestic and international spaceflight research, understanding behavioral adaptation to the space environment for extended durations is scant. Here we report the first detailed behavioral analysis of mice flown in the NASA Rodent Habitat on the International Space Station (ISS). Following 4-day transit from Earth to ISS, video images were acquired on orbit from 16- and 32-week-old female mice. Spaceflown mice engaged in a full range of species-typical behaviors. Physical activity was greater in younger flight mice as compared to identically-housed ground controls, and followed the circadian cycle. Within 7-10 days after launch, younger (but not older), mice began to exhibit distinctive circling or 'race-tracking' behavior that evolved into coordinated group activity. Organized group circling behavior unique to spaceflight may represent stereotyped motor behavior, rewarding effects of physical exercise, or vestibular sensation produced via self-motion. Affording mice the opportunity to grab and run in the RH resembles physical activities that the crew participate in routinely. Our approach yields a useful analog for better understanding human responses to spaceflight, providing the opportunity to assess how physical movement influences responses to microgravity.

| S-EPMC6459880 | biostudies-literature

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-07-26 | GSE110563 | GEO

Successful amplification of DNA aboard the International Space Station.

Project description:As the range and duration of human ventures into space increase, it becomes imperative that we understand the effects of the cosmic environment on astronaut health. Molecular technologies now widely used in research and medicine will need to become available in space to ensure appropriate care of astronauts. The polymerase chain reaction (PCR) is the gold standard for DNA analysis, yet its potential for use on-orbit remains under-explored. We describe DNA amplification aboard the International Space Station (ISS) through the use of a miniaturized miniPCR system. Target sequences in plasmid, zebrafish genomic DNA, and bisulfite-treated DNA were successfully amplified under a variety of conditions. Methylation-specific primers differentially amplified bisulfite-treated samples as would be expected under standard laboratory conditions. Our findings establish proof of concept for targeted detection of DNA sequences during spaceflight and lay a foundation for future uses ranging from environmental monitoring to on-orbit diagnostics.

| S-EPMC5691047 | biostudies-literature

Cardiovascular progenitor cells cultured aboard the International Space Station exhibit altered developmental and functional properties.

Project description:The heart and its cellular components are profoundly altered by missions to space and injury on Earth. Further research, however, is needed to characterize and address the molecular substrates of such changes. For this reason, neonatal and adult human cardiovascular progenitor cells (CPCs) were cultured aboard the International Space Station. Upon return to Earth, we measured changes in the expression of microRNAs and of genes related to mechanotransduction, cardiogenesis, cell cycling, DNA repair, and paracrine signaling. We additionally assessed endothelial-like tube formation, cell cycling, and migratory capacity of CPCs. Changes in microRNA expression were predicted to target extracellular matrix interactions and Hippo signaling in both neonatal and adult CPCs. Genes related to mechanotransduction (YAP1, RHOA) were downregulated, while the expression of cytoskeletal genes (VIM, NES, DES, LMNB2, LMNA), non-canonical Wnt ligands (WNT5A, WNT9A), and Wnt/calcium signaling molecules (PLCG1, PRKCA) was significantly elevated in neonatal CPCs. Increased mesendodermal gene expression along with decreased expression of mesodermal derivative markers (TNNT2, VWF, and RUNX2), reduced readiness to form endothelial-like tubes, and elevated expression of Bmp and Tbx genes, were observed in neonatal CPCs. Both neonatal and adult CPCs exhibited increased expression of DNA repair genes and paracrine factors, which was supported by enhanced migration. While spaceflight affects cytoskeletal organization and migration in neonatal and adult CPCs, only neonatal CPCs experienced increased expression of early developmental markers and an enhanced proliferative potential. Efforts to recapitulate the effects of spaceflight on Earth by regulating processes described herein may be a promising avenue for cardiac repair.

| S-EPMC6062551 | biostudies-literature

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