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ABSTRACT: Maize carbonic anhydrase mutants at high and low CO2

PROVIDER: PRJNA436918 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6805470.fastq.gz	Fastqsanger.gz
	SRR6805471.fastq.gz	Fastqsanger.gz
	SRR6805472.fastq.gz	Fastqsanger.gz
	SRR6805473.fastq.gz	Fastqsanger.gz
	SRR6805474.fastq.gz	Fastqsanger.gz

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Project description:Maize carbonic anhydrase mutants at high and low CO2

| PRJNA436918 | ENA

Global transcriptomic changes following elevated carbon dioxide (5%) in the wild-type (WT) and mutant (M2; RNAi-knockdown line of carbonic anhydrase (CA2)) Nannochloropsis oceanica IMET1: Illumina sequencing

Project description:In Nannochloropsis oceanica IMET1, transcript knockdown of a cytosolic carbonic anhydrase (CA2; g2018) specifically inhibited by HC resulted in ~45%, ~30% and ~40% elevation of photosynthetic oxygen evolution rate, growth rate and biomass accumulation rate under high CO2 (5% ), respectively. This CA2-knockdown mutant is demonated as M2. To probe mechanistic links underlying the mutant (M2; RNAi-knockdown line of carbonic anhydrase (CA2)) phenotypes, temporal transcriptomic profiles are compared between RNAi-knockdown line of carbonic anhydrase (CA2) and WT, at 12 h and 24h under high CO2 (5%).

2020-10-28 | GSE115445 | GEO

LC-MS/MS to identify proteins present in apoplastic fluid extracted from WT and ca1ca4 mutant Arabidopsis plants grown under low and high CO2 conditions

Project description:geLC-MS/MS analysis was performed to identify proteins that are present in apoplastic fluid isolated from rosette leaves of 8-week-old wild type and carbonic anhydrase (ca1ca4) mutant Arabidopsis. Plants were grown in either low (150 ppm) or high (500 ppm) CO2.

2014-08-22 | PXD000693 | Pride

Transcriptional profiling of Brucella ovis strains cultivated in the presence of high (5%) and low (0.04%) CO2

Project description:Wild-type Brucella ovis ATCC 25840 requires the addition of 5% CO2 to the atmosphere to grow on either nutrient agar plates or in liquid broth culture. The goal of this study was to measure the transcriptional response of two Brucella ovis strains under high (5%) and low (0.04%) CO2. The two strains assayed were 1) wild-type and 2) a spontaneous mutant that can be cultivated in standard atmospheric levels of CO2 (~0.04%). Wild-type B. ovis harbors a single nucleotide insertion at the 3' end of a beta carbonic anhydrase gene, bcaA, which renders it non-functional. The spontaneous mutant lost this nucleotide insertion, which restored the consensus reading frame and results in a functional BcaA protein.

2019-05-04 | GSE130678 | GEO

carbonic anhydrase (1 or 2) simple or double mutants-Comparative transcriptome analysis between carbonic anhydrase mutants and wild type Arabidopsis

Project description:cea05-01_carbonic-anhydrase - carbonic anhydrase (1 or 2) simple or double mutants - Identification of genes differentially expressed in leaves of single CA1 and CA2 T-DNA insertional mutants and in the corresponding double mutant vs wild type - CA1 (At3g01500) and CA2 (At5g14740) T-DNA insertional mutant lines, the double (CA1+CA2) mutant and wild type Arabidopsis seeds were sown in soil in a phytotron. Leaves were harvested 40 days later for RNA extraction

2012-03-25 | GSE36625 | GEO

A mechanistic linkage between low CO2 and evolution of C4 photosynthesis

Project description:Physiological and molecular evidences have shown earlier that low CO2 might have been a major driving force during the evolution of C4 photosynthesis, not only being a selection pressure but also as a signaling agent. However, a mechanistic linkage between low CO2 and C4 emergence is missing. In this study, using transcriptomics study in model plant Arabidopsis thaliana, we demonstrated that under long-term low CO2 treatments, the up-regulation of C4 related genes were linked to the up-regulation of genes involved in photorespiration, nitrogen assimilation and glycolysis. Plants under low CO2 also showed altered d13C. The carbonic anhydrase (CA), phosphoenolpyruvate carboxylase (PEPC), malate dehydrogenase (MDH), glutamine oxoglutarate aminotransferase (GS-GOGAT), which are required to reassimilate ammonia, were up-regulated under low CO2. Furthermore, under low CO2, genes involved in PEP regeneration from glycolysis were up-regulated while pyruvate orthophosphate dikinase (PPDK) was down-regulated, suggesting a route of PEP regeneration from glycolysis. All these results suggested that under long-term low CO2 treatment, the selection pressure to recapture the released ammonia from the increased photorespiration might have promoted the evolution of mechanisms for generation PEP from glycolysis and enhancement of enzymes catalyzing formation of oxaloacetate, one intermediate which can accept ammonia residue. These adjustments in metabolism provide a mechanistic linkage between low CO2 and evolution of C4 photosynthesis.

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Project description:carbonic anhydrase (1 or 2) simple or double mutants-Comparative transcriptome analysis between carbonic anhydrase mutants and wild type Arabidopsis

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carbonic anhydrase (1 or 2) simple or double mutants-Comparative transcriptome analysis between carbonic anhydrase mutants and wild type Arabidopsis

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Project description:Carbonic anhydrase IX (CA 9) is a transmembrane isoform of carbonic anhydrase (CA) that contributes to an acidification of tumor microenvironment. The expression of CA 9 in cervical tumors was shown to be strongly involved in high incidence of metastasis and poor prognosis. To search for the key regulators of metastasis related to ectopic expression of CA 9, we investigated differentially expressed gene profiles in CA 9- transfected cervix carcinoma cell line C33-A (CF) compared with mock-transfected (CP) cell line, using Affymetrix Human Genome U133 Plus 2.0 Array.

2010-03-02 | GSE20569 | GEO

High CO2- and pathogen-driven expression of carbonic anhydrase βCA3 confers basal immunity in tomato

Project description:High CO2- and pathogen-driven expression of carbonic anhydrase βCA3 confers basal immunity in tomato

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