Project description:Global warming has shifted climate zones poleward or upward. However, understanding the responses and mechanism of microbial community structure and functions relevant to natural climate zone succession is challenged by the high complexity of microbial communities. Here, we examined soil microbial community in three broadleaved forests located in the Wulu Mountain (WLM, temperate climate), Funiu Mountain (FNM, at the border of temperate and subtropical climate zones), or Shennongjia Mountain (SNJ, subtropical climate).Soils were characterized for geochemistry, Illumina sequencing was used to determine microbial taxonomic communities and GeoChips 5.0 were used to determine microbial functional genes.
Project description:There are 10 mice in the experiment, named REC. The mice were fed with high salt diets (5% NaCl) for 4 weeks and then fed with normal salt diets for 4 weeks. Then extracted DNA from mice gastric flora to detect changes in the gastric flora of mice.
Project description:Continuous cell lines are important research tools in biology and medicine. However, literature demonstrates that over-subculturing changes cell lines properties over time. In addition, cultured cells adapt to stress and confusion by evolution - both genotypic and phenotypic. The aim of this study is to identify differentially expressed genes and key biological pathways at high-passage cells compared to lower-passage cells. To do that, we performed microarray analyses on NR8383 macrophages at 20 and 200 passages numbers. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 686098
2021-07-23 | GSE117487 | GEO
Project description:High-throughput sequencing of zerbafish intestinal flora
Project description:To explore the regulatory mechanism of intestinal flora in Citrobacter rodentium -induced intestinal infection by transcriptome analysis at miRNA molecular level.
Project description:We used in silico analyses to identify the distinct transcriptome signatures of two populations living at high altitudes and identified potential mechanisms that underlie high-altitude adaptation. Data generated in this study indicate that placentas from Tibetan women are genetically distinct from European women at high altitudes, and appear to be protected from hypoxia and stress.
Project description:Divided into four groups,CTL,Salt,(S+B),B.CTL group was fed with normal salt diets for 4 weeks,Salt group were fed with high salt diets(5% NaCl)for 4 weeks,(S+B)group were fed with high salt diets mixed with buckwheat (1:1)for 4 weeks ,B group: first fed high salt diets for 4 weeks and then fed with buckwheat for 4 weeks. Extract mouse gastric flora DNA and sequence.
