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Next Generation Sequencing of LdlrKO LXRα-phosphorylation disrupted macrophage transcriptomes

ABSTRACT: Next Generation Sequencing of LdlrKO LXRα-phosphorylation disrupted macrophage transcriptomes

PROVIDER: PRJNA445274 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing of LdlrKO LXRα-phosphorylation disrupted macrophage transcriptomes

Project description:Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. Besides ligand binding, LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess changes in bone marrow derived macrophage transcriptional profiles of mice that carry LysMcre directed phosphorylation deficient-version of LXRα compared (S196A) to wild-type (WT).

2018-06-29 | GSE112213 | GEO

Next Generation Sequencing of Wild Type and LXRα-Ser196 phosphorylation deficient Murine Hepatic Transcriptomes on a Chow diet

Project description:Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess changes in the hepatic transcriptional profiles of mice that carry a whole-body phosphorylation deficient knock in mutant of LXRα (S196A) compared to wild-type (WT) fed a chow diet.

2019-01-21 | GSE96650 | GEO

Next Generation Sequencing of Wild Type and LXRα-Ser196 phosphorylation deficient Murine Hepatic Transcriptomes on a High Fat/High Cholesterol Diet

Project description:Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess changes in the hepatic transcriptional profiles of mice that carry a whole-body phosphorylation deficient knock in mutant of LXRα (S196A) compared to wild-type (WT) upon being fed a HFHC diet.

2019-01-21 | GSE95359 | GEO

Impaired Liver X Receptor alpha phosphorylation affects hepatic chromatin H3K27 acetylation patterns

Project description:Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. Besides ligand binding, LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess how changes in the phosphorylation status of LXRα affect the H3K27Ac histone modification, a known marker for active regulatory elements in promoters and enhancers, in livers of mice mice that carry a whole-body phosphorylation deficient-version of LXRα (S196A) compared to wild-type (WT), in response to a high fat-high cholesterol diet.

2019-01-22 | GSE114104 | GEO

Next-generation sequencing analysis of macrophage engulfing control or STC1-OE tumor corpses related transcriptomes

Project description:Purpose: To explore the transcriptomes changes in macrophage during long term engulfment of control or STC1-OE tumor corpses.

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Next Generation Sequencing Facilitates Quantitative Analysis of Peritoneal Macrophage Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Peritoneal Macrophage Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of sh-NC and sh-Etv5 macrophage line Raw264.7 Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare sh-NC with sh-Etv5 macrophage line Raw264.7 transcriptome profiling (RNA-seq).

2022-02-06 | GSE166294 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Ern1f/f and Ern1f/fLyz2-Cre macrophage Transcriptomes

Project description:Purpose: IRE1 are indicated to play important role in LPS-induced macrophage activation. The goals of this study are to investigate the effects of IRE1a abrogation in macrophages on Interleukin-4-induced alernative activation of macrophages. Methods: Total cellular RNAs of M2-polarized BMDMs by IL-4 treatment were isolated and subjected to commercial RNA-seq analysis. RNA-seq data were uniquely mapped to mm10 reference genome by TopHat v1.4.1. Expression values were assigned to gene level by Cufflinks v1.3.0. Results: Map ratios of all RNA-seq samples were above 80%. Merged expression values of all samples included 10,863 genes. Principle component analysis showed distinguished patterns between groups. Conclusions: Our study represents detailed analysis of transcriptomes of IL-4-activated macrophages, with biologic replicates, generated by RNA-seq technology. Based on the analysis of our RNA-seq data, increased expression levels of marker genes of macrophage alternative activation were found in IRE1a-abrogated macrophages copared to control cells. Our results demonstrates the loss of ER stress sensor IRE1a in macrophages enhanced IL-4-induced alternative activation.

2017-03-28 | GSE94659 | GEO

Macrophage-enriched Sectm1a promotes efficient efferocytosis to attenuate ischemia/reperfusion-induced cardiac injury

Project description:Efficient clearance and degradation of apoptotic cardiomyocytes by macrophages (termed efferocytosis) are critical for inflammation resolution and restoration of cardiac function after myocardial ischemia/reperfusion (I/R). Here, we define secreted and transmembrane protein 1a (Sectm1a), a cardiac macrophage-enriched gene, as a modulator of macrophage efferocytosis in I/R hearts. Upon myocardial I/R, Sectm1a-KO mice exhibit impaired macrophage efferocytosis, leading to massive accumulation of apoptotic cardiomyocytes, cardiac inflammation, fibrosis, and consequently, exaggerated cardiac dysfunction. By contrast, therapeutic administration of recombinant SECTM1A protein significantly enhances macrophage efferocytosis and improves cardiac function. Mechanistically, SECTM1A can elicit autocrine effects on the activation of glucocorticoid-induced TNF receptor (GITR) at the surface of macrophages, leading to the upregulation of liver X receptor alpha (LXRα) and its downstream efferocytosis-related genes and lysosomal enzyme genes. Our study suggests that Sectm1a-mediated activation of Gitr/LXRα axis could be a promising approach to enhance macrophage efferocytosis for the treatment of myocardial I/R injury.

2024-01-23 | GSE253579 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and dek35 mutant Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that dek35 mutant influence the expression of mitochondrial oxidative phosphorylation associated genes. Results provide important information of the gene expression level regulation of mitochondrial complexes assembly proteins, translational elongation factors and genes associated with other biological processes. Endosperm mRNA profiles of 18DAP wild type (WT) and dek35 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

2016-03-25 | E-GEOD-79583 | biostudies-arrayexpress

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