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Gene expression profile of SmarT-cells compared to control 1G CAR T cells

ABSTRACT: Gene expression profile of SmarT-cells compared to control 1G CAR T cells

PROVIDER: PRJNA451006 | ENA |

REPOSITORIES: ENA

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Gene expression profile of SmarT-cells compared to control 1G CAR T cells

Project description:The objective of this experiment was to compare the transcriptional profile of T cells modified to express CAR, TBBR and 4/7 ICR (i.e. SmarT-cells) against control 1G CAR T cells expressing CD3z endodomain. RNA samples were isolated from trangenic T cells generated from three independent donors.

2018-04-20 | GSE113391 | GEO

Gene expression profile of SmarT-cells compared to control T cells modified to express ΔCAR, ΔTGFbRII and ΔIL4R

Project description:The objective of this experiment was to compare the transcriptional profile of T cells modified to express CAR, TBBR and 4/7 ICR (i.e. SmarT-cells) against control T cells expressing the delta constructs (i.e. ΔCAR, ΔTGFbRII and ΔIL4R). RNA samples were isolated from trangenic T cells generated from three independent donors.

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Gene expression profile of CAR T cells compared to control ΔCAR T cells

Project description:Gene expression profile of CAR T cells compared to control ΔCAR T cells

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Gene expression profile of SmarT-cells compared to control T cells modified to express ΔCAR, ΔTGFbRII and ΔIL4R

Project description:Gene expression profile of SmarT-cells compared to control T cells modified to express ΔCAR, ΔTGFbRII and ΔIL4R

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Gene expression profile of CAR T cells compared to control ΔCAR T cells

Project description:The objective of this experiment was to compare the transcriptional profile of CAR (CD3z endodomain) vs control ΔCAR (truncated version that lacks an endodomain) modified T cells. RNA samples were isolated from trangenic T cells generated from three independent donors.

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Compared the gene expression profiles between the PTL-Her2-CAR-T cells and Her2-CAR-T cells isolated from tumor tissues

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RNA seq analysis of T cells，EpCAM CAR-T cells and rapamycin-pretreated EpCAM CAR-T cells

Project description:Purpose: Compared the differences in the transcriptome of T cells versus EpCAM CAR-T cells and EpCAM CAR-T cells versus rapamycin-pretreated EpCAM CAR-T cells.Methods: Next-generation sequencing (NGS) has revolutionized the systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) between T cells and EpCAM CAR-T cells，and between EpCAM CAR-T cells and rapamycin-pretreated EpCAM CAR-T cells. Results: RNA-seq showed that CAR-T cells significantly upregulated the expression of exhaustion markers compared to T cells. Gene Set Enrichment Analysis (GSEA) analysis showed that CAR-T cells cells were enriched for signatures of apoptosis. Compared with T cells, Kyoto Encyclopedia of Genes and Genomessignaling(KEGG) pathway analysis showed that the PI3K/AKT/mTOR signaling pathway was significantly enriched. Likewise, GSEA showed that the mTOR signaling pathway was significantly upregulated in CAR-T cells. Next, we analyzed the RNA-seq data between CAR-T cells and rapamycin-pretreated CAR-T cells. RNA-seq showed that rapamycin-treated CAR T down-regulated the expression of exhaustion markers compared with untreated CAR-T cells. GSEA analysis showed that the apoptosis-related gene set was enriched in untreated CAR-T cells.Conclusions:In vitro culture promotes the terminal differentiation of CAR-T cells.CAR-T cells present a highly activated mTOR phenotype. Rapamycin pretreatment prevents CAR-T cells terminal differentiation.

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2019-03-29 | PXD012833 | Pride

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Project description:Adoptive transfer of chimeric antigen receptor (CAR)-T cells is expected to become the first line of treatment for multiple malignancies, following the enormous success of anti-CD19 therapies. However, their mechanism of action is not fully understood, and clear guidelines for the design of safe and efficient receptors are missing. We hereby describe a systematic analysis of the CAR “signalosome” in human primary T cells. Two CAR designs were compared: a second-generation (PSCA2) and a third-generation (PSCA3) anti-PSCA CAR. Phosphorylation events triggered by CAR-mediated recognition of target cells were quantified by mass spectrometry.

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