Project description:Paragonimiasis is a kind of food borne parasitic disease. Due to its lack of specificity, it is difficult to diagnose and treat early in clinic. It is often misdiagnosed as pulmonary tuberculosis and lung cancer, which has a great impact on public health. We established an animal model of Paragonimus proliferus infection in rats, obtained the different growth stages of Paragonimus proliferus and studied its proteomics. Objective is to explore the mechanism of immune evasion during the whole growth and development of Paragonimus proliferus.

2020-12-24 | PXD023304 | iProX

Transcriptome sequence of oriental melon fruit at diferent stages in two cultivars

Project description:Purpose: the goals of this study are to compare fruit of two clitivars oriental melon transcriptome profiling (RNA-seq) at different stages to explore carotenoid potentail carotenoid accumulation mechanism Methods:The transcriptome sequence of two cultivars oriental melon fruits at different stages were generated by deep sequencing with three repeats using Illumina. The sequence reads that passed filters were mapped to melon genome (http://cucurbitgenomics.org/organism/18) using HISAT2 software. The differently expressed genes were identify by |log2(FoldChange)| > 0 & padj <= 0.05, and qRT–PCR validation was performed using SYBR Green assays Result:Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the melon genome. The differentially expressed genes were functionally classified by GO and KEGG enrichment. We focused on carotenoid metabolism related gene and validated using qRT-PCR. The results showed RNA-seq and qRT-PCR were highly correlated. Conclusion: Our study provided transcriptome sequence of oriental melon fruits at different stages in two cultivars. The optimized data analysis workflows reported here should provide comparative framework of expression profiles. Our transcriptome characterization contribute to analyze gene functions and metabolic process of oriental melon.

2022-01-13 | GSE193412 | GEO

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