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Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells

ABSTRACT: Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells

PROVIDER: PRJNA470652 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7138671.fastq.gz	Fastqsanger.gz
	SRR7138672.fastq.gz	Fastqsanger.gz
	SRR7138673.fastq.gz	Fastqsanger.gz

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Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells

Project description:We compared the genome occupancy for FLAG-tagged versions of the ETS factors ERG and EHF in the normal prostate epithelial cell line RWPE1. Our in vitro binding studies support a model whereby oncogenic ETS factors like ERG bind cooperativly with AP1 factors at closly spaced ETS-AP1 sites, while certain non-oncogenic factors like EHF bind anti-cooperatively with AP1 at the same sites. ETS and AP1 binding motifs were enriched in both ChIP datasets, but the ERG-FLAG bound reginos contained a much higher percentage of ETS-AP1 sites spaced in close proximity, consistent with our in vitro binding data.

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