ERG-Proximity biotin labeling_2022-03-30
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: 
Brian Raught
PROVIDER: MSV000089158 | MassIVE | Wed Mar 30 06:27:00 BST 2022
SECONDARY ACCESSION(S): PXD032874
REPOSITORIES: MassIVE
Similar Datasets
Project description:We performed a BioID experiment in a HEK293 Flp-In T-Rex cell line that contains human GNL3 cDNA integrated at the FRT site tagged with BirA and FLAG under the control of a promoter regulated with doxycycline.
2023-12-05 | PXD045389 | Pride
Project description:BioID Controls for Flp-In T-REx HEK293 cells are the following;
Empty Vector:
VL_20151116_COT_01_HEK293_pcDNA5EV_HB
VL_20151116_COT_02_HEK293_pcDNA5EV_HB
VL_20151116_COT_03_HEK293_pcDNA5EV_HB
VL_20151116_COT_04_HEK293_pcDNA5EV_HB
BirA-Flag-EGFP:
VL_20150825_COT_05_HB
VL_20150825_COT_06_HB
VL_20150825_COT_07_HB
VL_20150825_COT_08_HB
BirA-Flag-EGFP-CAAX :
VL_20181019_COT_01_HB
VL_20181019_COT_02_HB
VL_20181019_COT_03_HB
VL_20181019_COT_04_HB
BioID samples for Flp-In T-REx HEK293 cells are the following;
RAC1_WT:
2186_ZL_RAC1_WT_BR1_Velos1_P103_HB
2308_ZL_RAC1_WT_BR2_Velos1_HB
RHOG_WT:
VL_20170123_COT_01_HB
VL_20170123_COT_02_HB
RHOA_WT:
VL_20160323_COT_04new2_HB
VL_20180104_COT_01_HB
CDC42_WT:
VL_20180104_COT_03_HB
VL_20180104_COT_04_HB
RAC1_G15A:
VL_20180105_COT_01_2_HB
VL_20180105_COT_02_HB
CDC42_G15A:
VL_20180105_COT_03_HB
VL_20180105_COT_04_HB
RHOG_G15A:
VL_20180108_COT_01_HB
VL_20180108_COT_02_HB
RHOA_G17A:
VL_20180108_COT_03_HB
VL_20180108_COT_04_HB
CDC42_G12V:
2189_ZL_CDC42_G12V_BR1_Velos1_P103_HB
2263_ZL_CDC42_G12V_BR2_Velos1_HB
RAC1_G12V:
1871_RAC1_G12V_BirA_Flag_HEK293_2-2_HB
VL_20150825_COT_02_HB
RAC2_G12V:
2199_ZL_RAC2_G12V_BR1_Velos1_P103_HB
2323_ZL_RAC2_G12V_BR2_Velos1_HB
RAC3_G12V:
2200_ZL_RAC3_G12V_BR1_Velos1_P103_HB
2321_ZL_RAC3_G12V_BR2_Velos1_HB
RHOA_G14V:
2190_ZL_RHOA_G14V_BR1_Velos1_P103_HB
2262_ZL_RHOA_G14V_BR2_Velos1_HB
RHOB_G14V:
2191_ZL_RHOB_G14V_BR1_Velos1_P103_HB
2261_ZL_RHOB_G14V_BR2_Velos1_HB
RHOBTB1_WT:
2201_ZL_RHOBTB1_WT_BR1_Velos1_P103_HB
2320_ZL_RHOBTB1_WT_BR2_Velos1_HB
RHOBTB2_WT:
2202_ZL_RHOBTB2_WT_BR1_Velos1_P103_HB
2318_ZL_RHOBTB2_WT_BR2_Velos1_HB
RHOC_G14V:
2192_ZL_RHOC_G14V_BR1_Velos1_P103_HB
2260_ZL_RHOC_G14V_BR2_Velos1_HB
RHOD_G26V:
2193_ZL_RHOD_G26V_BR1_Velos1_P103_HB
2259_ZL_RHOD_G26V_BR2_Velos1_HB
RHOF_G28V:
2246_ZL_RHOF_G28V_BR1_2194ReRun_Velos1_P103_HB
2258_ZL_RHOF_G28V_BR2_Velos1_HB
RHOG_G12V:
2306_ZL_RHOG_G12V_BR2_Velos1_HB
VL_20170123_COT_03_HB
RHOH_WT:
2203_ZL_RHOH_WT_BR1_Velos1_P103_HB
2317_ZL_RHOH_WT_BR2_Velos1_HB
RHOJ_G40V:
2195_ZL_RHOJ_G40V_BR1_Velos1_P103_HB
2257_ZL_RHOJ_G40V_BR2_Velos1_HB
RHOQ_G18V:
2196_ZL_RHOQ_G18V_BR1_Velos1_P103_HB
2338_ZL_RHOQ_G18V_BR2_Velos1_HB
RHOU_G58V:
2250_ZL_RHOU_G58v_BR1_2197ReRun_Velos1_P103_HB
2337_ZL_RHOU_G58V_BR2_Velos1_HB
RHOV_G40V:
2198_ZL_RHOV_G40V_BR1_Velos1_P103_HB
2324_ZL_RHOV_G40V_BR2_Velos1_HB
RND1_WT:
2249_ZL_RND1_WT_BR1_Velos1_P103_HB
2313_ZL_RND1_WT_BR2_Velos1_HB
RND2_WT:
2247_ZL_RND2_WT_BR1_Velos1_P103_HB
2315_ZL_RND2_WT_BR2_Velos1_HB
RND3_WT:
VL_20151116_COT_05_HB
VL_20151116_COT_06_HB
BioID Controls for Flp-In T-REx HeLa cells are the following;
Empty Vector:
QE_20180917_COT_01_HB
QE_20180917_COT_02_HB
QE_20180917_COT_03_HB
QE_20180917_COT_04_HB
BirA-Flag-EGFP:
QE_20151030_COT_02_HB
QE_20151030_COT_03_HB
QE_20151030_COT_04_HB
QE_20151030_COT_05_HB
BirA-Flag-EGFP-CAAX:
QE_20181022_COT_01_HB
QE_20181022_COT_02_HB
QE_20181022_COT_03_HB
QE_20181022_COT_04_HB
BioID samples for Flp-In T-REx HeLa cells are the following;
CDC42_G15A:
QE_20171218_COT_05_HB
QE_20171218_COT_06_HB
CDC42_WT:
QE_20171218_COT_01_HB
QE_20171218_COT_02_HB
RAC1_G15A:
QE_20171218_COT_03_HB
QE_20171218_COT_04_HB
RAC1_WT:
QE_20151102_COT_01_HB
QE_20151102_COT_02_HB
RHOA_G17A:
QE_20171218_COT_07_HB
QE_20171218_COT_08_HB
RHOA_WT:
QE_20171201_COT_07_HB
QE_20171201_COT_08_HB
RHOG_G15A:
QE_20171220_COT_01_HB
QE_20171220_COT_02_HB
RHOG_WT:
QE_20161215_COT_01_HB
QE_20161215_COT_02_HB
CDC42_G12V:
QE_20171201_COT_09_HB
QE_20171201_COT_10_HB
RAC1_G12V:
QE_20151030_COT_06_HB
QE_20151030_COT_07_HB
RAC2_G12V:
QE_20180105_COT_05_HB
QE_20180105_COT_06_HB
RAC3_G12V:
QE_20180105_COT_07_HB
QE_20180105_COT_08_HB
RHOA_G14V:
QE_20171205_COT_17_HB
QE_20171205_COT_18_HB
RHOB_G14V:
QE_20180105_COT_01_HB
QE_20180105_COT_02_HB
RHOBTB1_WT:
QE_20180108_COT_07_HB
QE_20180108_COT_08_HB
RHOBTB2_WT:
QE_20171205_COT_19_HB
QE_20171205_COT_20_HB
RHOC_G14V:
QE_20180105_COT_03_HB
QE_20180105_COT_04_HB
RHOD_G26V:
QE_20180108_COT_05_HB
QE_20180108_COT_06_HB
RHOF_G28V:
QE_20171130_COT_05_HB
QE_20171130_COT_06_HB
RHOG_G12V:
QE_20161215_COT_03_HB
QE_20161215_COT_04_HB
RHOH_WT:
QE_20171204_COT_15_HB
QE_20171204_COT_16_HB
RHOJ_G40V:
QE_20171130_COT_03_HB
QE_20171130_COT_04_HB
RHOQ_G18V:
QE_20180105_COT_09_HB
QE_20180105_COT_10_HB
RHOU_G58V:
QE_20171204_COT_11_HB
QE_20171204_COT_12_new_HB
RHOV_G40V:
QE_20171130_COT_01_HB
QE_20171130_COT_02_HB
RND1_WT:
QE_20180108_COT_01_HB
QE_20180108_COT_02_HB
RND2_WT:
QE_20171204_COT_13_HB
QE_20171204_COT_14_HB
RND3_WT:
QE_20180108_COT_03_HB
QE_20180108_COT_04_HB
2019-10-20 | MSV000084477 | MassIVE
Project description:BioID Controls for Flp-In T-REx HEK293 cells are the following;
Empty Vector:
VL_20151116_COT_01_HEK293_pcDNA5EV_HB
VL_20151116_COT_02_HEK293_pcDNA5EV_HB
VL_20151116_COT_03_HEK293_pcDNA5EV_HB
VL_20151116_COT_04_HEK293_pcDNA5EV_HB
BirA-Flag-EGFP:
VL_20150825_COT_05_HB
VL_20150825_COT_06_HB
VL_20150825_COT_07_HB
VL_20150825_COT_08_HB
BirA-Flag-EGFP-CAAX:
VL_20181019_COT_01_HB
VL_20181019_COT_02_HB
VL_20181019_COT_03_HB
VL_20181019_COT_04_HB
BioID samples for Flp-In T-REx HEK293 cells are the following;
RAC1_WT:
2186_ZL_RAC1_WT_BR1_Velos1_P103_HB
2308_ZL_RAC1_WT_BR2_Velos1_HB
RHOG_WT:
VL_20170123_COT_01_HB
VL_20170123_COT_02_HB
RHOA_WT:
VL_20160323_COT_04new2_HB
VL_20180104_COT_01_HB
CDC42_WT:
VL_20180104_COT_03_HB
VL_20180104_COT_04_HB
RAC1_G15A:
VL_20180105_COT_01_2_HB
VL_20180105_COT_02_HB
CDC42_G15A:
VL_20180105_COT_03_HB
VL_20180105_COT_04_HB
RHOG_G15A:
VL_20180108_COT_01_HB
VL_20180108_COT_02_HB
RHOA_G17A:
VL_20180108_COT_03_HB
VL_20180108_COT_04_HB
CDC42_G12V:
2189_ZL_CDC42_G12V_BR1_Velos1_P103_HB
2263_ZL_CDC42_G12V_BR2_Velos1_HB
RAC1_G12V:
1871_RAC1_G12V_BirA_Flag_HEK293_2-2_HB
VL_20150825_COT_02_HB
RAC2_G12V:
2199_ZL_RAC2_G12V_BR1_Velos1_P103_HB
2323_ZL_RAC2_G12V_BR2_Velos1_HB
RAC3_G12V:
2200_ZL_RAC3_G12V_BR1_Velos1_P103_HB
2321_ZL_RAC3_G12V_BR2_Velos1_HB
RHOA_G14V:
2190_ZL_RHOA_G14V_BR1_Velos1_P103_HB
2262_ZL_RHOA_G14V_BR2_Velos1_HB
RHOB_G14V:
2191_ZL_RHOB_G14V_BR1_Velos1_P103_HB
2261_ZL_RHOB_G14V_BR2_Velos1_HB
RHOBTB1_WT:
2201_ZL_RHOBTB1_WT_BR1_Velos1_P103_HB
2320_ZL_RHOBTB1_WT_BR2_Velos1_HB
RHOBTB2_WT:
2202_ZL_RHOBTB2_WT_BR1_Velos1_P103_HB
2318_ZL_RHOBTB2_WT_BR2_Velos1_HB
RHOC_G14V:
2192_ZL_RHOC_G14V_BR1_Velos1_P103_HB
2260_ZL_RHOC_G14V_BR2_Velos1_HB
RHOD_G26V:
2193_ZL_RHOD_G26V_BR1_Velos1_P103_HB
2259_ZL_RHOD_G26V_BR2_Velos1_HB
RHOF_G28V:
2246_ZL_RHOF_G28V_BR1_2194ReRun_Velos1_P103_HB
2258_ZL_RHOF_G28V_BR2_Velos1_HB
RHOG_G12V:
2306_ZL_RHOG_G12V_BR2_Velos1_HB
VL_20170123_COT_03_HB
RHOH_WT:
2203_ZL_RHOH_WT_BR1_Velos1_P103_HB
2317_ZL_RHOH_WT_BR2_Velos1_HB
RHOJ_G40V:
2195_ZL_RHOJ_G40V_BR1_Velos1_P103_HB
2257_ZL_RHOJ_G40V_BR2_Velos1_HB
RHOQ_G18V:
2196_ZL_RHOQ_G18V_BR1_Velos1_P103_HB
2338_ZL_RHOQ_G18V_BR2_Velos1_HB
RHOU_G58V:
2250_ZL_RHOU_G58v_BR1_2197ReRun_Velos1_P103_HB
2337_ZL_RHOU_G58V_BR2_Velos1_HB
RHOV_G40V:
2198_ZL_RHOV_G40V_BR1_Velos1_P103_HB
2324_ZL_RHOV_G40V_BR2_Velos1_HB
RND1_WT:
2249_ZL_RND1_WT_BR1_Velos1_P103_HB
2313_ZL_RND1_WT_BR2_Velos1_HB
RND2_WT:
2247_ZL_RND2_WT_BR1_Velos1_P103_HB
2315_ZL_RND2_WT_BR2_Velos1_HB
RND3_WT:
VL_20151116_COT_05_HB
VL_20151116_COT_06_HB
BioID Controls for Flp-In T-REx HeLa cells are the following;
Empty Vector:
QE_20180917_COT_01_HB
QE_20180917_COT_02_HB
QE_20180917_COT_03_HB
QE_20180917_COT_04_HB
BirA-Flag-EGFP:
QE_20151030_COT_02_HB
QE_20151030_COT_03_HB
QE_20151030_COT_04_HB
QE_20151030_COT_05_HB
BirA-Flag-EGFP-CAAX:
QE_20181022_COT_01_HB
QE_20181022_COT_02_HB
QE_20181022_COT_03_HB
QE_20181022_COT_04_HB
BioID samples for Flp-In T-REx HeLa cells are the following;
CDC42_G15A:
QE_20171218_COT_05_HB
QE_20171218_COT_06_HB
CDC42_WT:
QE_20171218_COT_01_HB
QE_20171218_COT_02_HB
RAC1_G15A:
QE_20171218_COT_03_HB
QE_20171218_COT_04_HB
RAC1_WT:
QE_20151102_COT_01_HB
QE_20151102_COT_02_HB
RHOA_G17A:
QE_20171218_COT_07_HB
QE_20171218_COT_08_HB
RHOA_WT:
QE_20171201_COT_07_HB
QE_20171201_COT_08_HB
RHOG_G15A:
QE_20171220_COT_01_HB
QE_20171220_COT_02_HB
RHOG_WT:
QE_20161215_COT_01_HB
QE_20161215_COT_02_HB
CDC42_G12V:
QE_20171201_COT_09_HB
QE_20171201_COT_10_HB
RAC1_G12V:
QE_20151030_COT_06_HB
QE_20151030_COT_07_HB
RAC2_G12V:
QE_20180105_COT_05_HB
QE_20180105_COT_06_HB
RAC3_G12V:
QE_20180105_COT_07_HB
QE_20180105_COT_08_HB
RHOA_G14V:
QE_20171205_COT_17_HB
QE_20171205_COT_18_HB
RHOB_G14V:
QE_20180105_COT_01_HB
QE_20180105_COT_02_HB
RHOBTB1_WT:
QE_20180108_COT_07_HB
QE_20180108_COT_08_HB
RHOBTB2_WT:
QE_20171205_COT_19_HB
QE_20171205_COT_20_HB
RHOC_G14V:
QE_20180105_COT_03_HB
QE_20180105_COT_04_HB
RHOD_G26V:
QE_20180108_COT_05_HB
QE_20180108_COT_06_HB
RHOF_G28V:
QE_20171130_COT_05_HB
QE_20171130_COT_06_HB
RHOG_G12V:
QE_20161215_COT_03_HB
QE_20161215_COT_04_HB
RHOH_WT:
QE_20171204_COT_15_HB
QE_20171204_COT_16_HB
RHOJ_G40V:
QE_20171130_COT_03_HB
QE_20171130_COT_04_HB
RHOQ_G18V:
QE_20180105_COT_09_HB
QE_20180105_COT_10_HB
RHOU_G58V:
QE_20171204_COT_11_HB
QE_20171204_COT_12_new_HB
RHOV_G40V:
QE_20171130_COT_01_HB
QE_20171130_COT_02_HB
RND1_WT:
QE_20180108_COT_01_HB
QE_20180108_COT_02_HB
RND2_WT:
QE_20171204_COT_13_HB
QE_20171204_COT_14_HB
RND3_WT:
QE_20180108_COT_03_HB
QE_20180108_COT_04_HB
2019-03-21 | MSV000083608 | MassIVE
Project description:BioID analysis was performed on wild type NHLRC2 and NHLRC2 containing a FINCA disease variant (pAsp148Tyr) tagged C-terminally with BirA-FLAG. Stable cells with the genomic insertion of recombinant wild type NHLRC2 and FINCA variant were generated as Flp-In 293 T-REx HEK 293 cell pools and expression of the recombinant protein was induced using tetracycline 24h before sample collection. The samples were collected as triplicates. The biotinylated proteins were isolated using Strep-Tactin beads and analysed using liquid chromatography-MS).
2024-06-07 | MSV000094956 | MassIVE
Project description:Determination of the interactome of different human FLAG-HA-tagged FAM98 paralogs (FAM98A, FAM98B and FAM98C) by co-immunoprecipitation/MS. Two expression constructs were used per paralog: one with an N-terminal and one with a C-terminal FLAG-HA-tag. Expression constructs were stably integrated into HEK Flp-In T-REx 293 cells and induced by addition of doxycycline. HEK Flp-In T-REx 293 cells without any integrated construct were used as control.
2026-06-08 | PXD076703 | Pride
Project description:To gain insights into the interactome of wild-type (WT) and S102P mutant GATAD1, we utilized the BioID method, which enables the study of protein-protein interactions. Specifically, we performed BioID proximity labeling experiments in stable Flp-In cells expressing different GATAD1 variants fused to BirA*_FLAG. These variants included BirA*_FLAG_GATAD1-WT, BirA*_FLAG_GATAD1-S102P, BirA*_FLAG_GATAD1-S102D, and BirA*_FLAG_GATAD1-S102A. By employing this approach, we aimed to characterize the protein interactors associated with these GATAD1 variants and gain insights into the functional consequences of the S102P mutation.
2024-02-15 | PXD043789 | Pride
Project description:Data contains LC-MS results of proximity-dependent biotin labeling to generate the protein interaction network of IRS1 protein fused with Flag-miniTurbo (N-terminal tag)
and expressed in T-REx HEK293 cells.
| MSV000088166 | MassIVE
Project description:Here we generated tetracycline inducible LRP6-BirA fusion lines in T-REx FlpIn cell lines for BioID proximity labelling proteomics experiments. We compare wt and mutant (YYYF>A mutant; mutates AP2 binding motifs to alanine) LRP6 binding partners following 12hrs Biotin labelling and Wnt3a treatment.
2025-04-30 | PXD054245 | Pride
Project description:Data deposited contain results from proximity-dependent biotinylation LC-MS (BioID) experiments in which T-REx Flp-In HEK293 cells express SCARB1 protein fused to biotin carboxylase BirA.
2023-11-01 | MSV000093221 | MassIVE
Project description:triple SILAC phosphoproteomics experiment of Flp-In T-Rex HEK293 cells stably expressing either FLAG empty, PINK1-FLAG Kinase dead or PINK1-FLAG wild type were grown in ‘light’ (K0R0), ‘medium’ (K4R6) and ‘heavy’ (K8,R10) SILAC media, respectively.
2015-09-16 | PXD002127 | Pride