Project description:The goal of the study was to test whether CBD103 genotype of North American gray wolves impacts the gene expression response to polyI:C or to live canine distemper virus. We established 24 primary cultures of epidermal keratinocytes from skin punches of North American gray wolves, and also generated an immortalized keratinocyte line and a CRISPR/Cas9 edited cell line. We evaluated the gene expression response of cells to either 24 hours challenge with 1 ug/ml polyI:C or to five days challenge with live canine distemper virus (100 TCID50/ml). Every challenged cell culture had a paired null control sample (plated and collected at same time points).
Project description:Purpose: The goal of the current study was to find the candidate genes responsible for the habita specific clock variation in N. discreta. Methods: We performed RNA-seq experiment using four strains ; African parent (FGSC8831), North American parent (FGSC 8578) and two representative progeny representing African clock phenotype (N309-89) and North American clock phenotype (N309-50). Results: We identified one candidate gene that meets our criteria; confirmed it's expression by qPCR and it's expression pattern is associated with parent genotype. Conclusions: Our approach using the expression profiles and SNP data of two parents and two representative progeny led us to identify a candidate gene for a complex clock adaptation phenotype.
Project description:This study was to collect expression level polymorphism (ELP) data for the barley genotypes Steptoe and Morex, parents of a widely used North American mapping population. Primarily, the data collected will show the amount of variation between these genotypes in seedling leaves grown in a greenhouse at the same time, with replications on three separate dates. ----------------------------------------------------------------------------------------------------------------------- Roger P. Wise(Head of Lab) and Stacy Barkeloo are the original contributors of this experimental data. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of them . The equivalent experiment is 'BB16' at PLEXdb. Keywords: genotype
Project description:This study was to collect expression level polymorphism (ELP) data for the barley genotypes Steptoe and Morex, parents of a widely used North American mapping population. Primarily, the data collected will show the amount of variation between these genotypes in seedling leaves grown in a greenhouse at the same time, with replications on three separate dates. -----------------------------------------------------------------------------------------------------------------------; Roger P. Wise(Head of Lab) and Stacy Barkeloo are the original contributors of this experimental data. PLEXdb(www.plexdb.org) has submitted this series at GEO on behalf of them . The equivalent experiment is 'BB16' at PLEXdb. Experiment Overall Design: genotype: Steptoe(3-replications); genotype: Morex(3-replications)
Project description:To study the population genetics context of the Saqqaq individual we carried out Illumina Bead-Array-based genotyping on four native North American and twelve north Asian populations.
Project description:Array CGH analysis of Helicobacter pylori strains isolated from a North American cohort of symptomatic pediatric patients. Keywords: genotyping_design
2009-11-05 | GSE13317 | GEO
Project description:Microbial communities within Microcystis colonies in North American lakes
| PRJNA778014 | ENA
Project description:Genotype-by-sequencing reads from Setophaga townsendi and related taxa
Project description:Environmental enteric dysfunction (EED) is a major impediment to the Sustainable Development Goals of improved childhood survival and healthy growth worldwide. Few studies have directly examined the affected intestine, limiting the development of effective interventions. The Study of Environmental Enteropathy and Malnutrition (SEEM, Pakistan) followed 416 at-risk children prospectively from birth to 24 months of age in a rural district of Pakistan with a high prevalence of undernutrition. The duodenal genome-wide methylome and transcriptome was determined in 52 undernourished SEEM participants refractory to nutritional interventions and 42 North American healthy controls and celiac disease patients. Biomarkers were measured at 9 months and tested for association with growth at 24 months in training (n=166) and validation (n=84) groups within SEEM.
