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Gene expression response of wolf epidermal keratinocytes to PolyI:C and canine distemper virus

ABSTRACT: The goal of the study was to test whether CBD103 genotype of North American gray wolves impacts the gene expression response to polyI:C or to live canine distemper virus. We established 24 primary cultures of epidermal keratinocytes from skin punches of North American gray wolves, and also generated an immortalized keratinocyte line and a CRISPR/Cas9 edited cell line. We evaluated the gene expression response of cells to either 24 hours challenge with 1 ug/ml polyI:C or to five days challenge with live canine distemper virus (100 TCID50/ml). Every challenged cell culture had a paired null control sample (plated and collected at same time points).

ORGANISM(S): Canis lupus

PROVIDER: GSE163163 | GEO | 2021/05/06

REPOSITORIES: GEO

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