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RNA-seq analysis for mRNA expression change in oxygen/glucose deprivation-treated cultured oligodendrocyte precursor cells

ABSTRACT: RNA-seq analysis for mRNA expression change in oxygen/glucose deprivation-treated cultured oligodendrocyte precursor cells

PROVIDER: PRJNA471909 | ENA |

REPOSITORIES: ENA

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RNA-seq analysis for mRNA expression change in oxygen/glucose deprivation-treated cultured oligodendrocyte precursor cells

Project description:Oligodendrocyte precursor cells (OPCs) regulate neuronal, glial and vascular systems in diverse ways and display phenotypic heterogeneity beyond their well-known role as a reservoir for mature oligodendrocytes. Here we demonstrated that perivascular OPCs in cerebral cortex were increased coupling with post-stroke angiogenesis in a mouse model of stroke induced by middle cerebral artery occlusion (MCAO). RNA-seq analysis revealed that primary cultured OPCs notably increased the gene expressions of numerous pro-angiogenic factors, while decreased those related to OPC maturation after oxygen glucose deprivation. The data showed that cerebral ischemia shifts the phenotype of OPCs to perivascular subtypes which can promote angiogenesis.

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RNA-seq analysis for mRNA expression change in alpha-synclein fibril-treated cultured oligodendrocyte precursor cells

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Investigation of Oligodendrocyte Precursor Cell Differentiation by Quantitative Proteomics

Project description:Oligodendrocytes, the myelinating cells of the central nervous system, are essential for correct brain function. They originate from oligodendrocyte precursor cells (OPCs) through a differentiation process which is only incompletely understood and impaired in a variety of demyelinating diseases. Better knowledge of this differentiation holds the promise to develop novel therapies for these disorders. We investigated the differentiation of rat oligodendrocyte precursor cells to oligodendrocytes in vitro. After confirmation of differentiation by immunohistochemical analysis using cell type-specific marker proteins, we conducted a quantitative proteomics study using tandem mass tags (TMT). We investigated four time points of differentiation covering early, intermediate, and late stages. Data analysis was perfomed using Mascot and Maxquant.

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Expression profiles in Chd7 knockdown oligodendrocyte precursor cells

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Single-cell transcriptomic analysis of human and mouse oligodendrocyte precursor cells

Project description:We report single cell gene expression analysis of human fetal and mouse postnatal oligodendrocyte precursor cells

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RNA-seq analysis for mRNA expression difference between male and female cultured oligodendrocyte precursor cells

Project description:The susceptibility to neurological and psychiatric disorders reveals sex-specific differences. Several studies have reported sex-related differences in the structure and function of human brains. Oligodendrocyte precursor cells (OPCs) regulate the neuronal system in various ways and play crucial roles in brain homeostasis besides their well-known role as a reservoir for mature oligodendrocytes. Here, we revealed transcriptomic differences in OPCs isolated from male and female neonatal rat brains.

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miRNA profile of microglial cells under oxygen-glucose deprivation

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RNA-seq analysis for mRNA expression change in alpha-synclein fibril-treated cultured oligodendrocyte precursor cells

Project description:RNA-seq analysis for mRNA expression change in alpha-synclein fibril-treated cultured oligodendrocyte precursor cells

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Reactive human iPSC astrocytes suppress oligodendrocyte precursor cell differentiation

Project description:Reactive human iPSC astrocytes suppress oligodendrocyte precursor cell differentiation

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