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Bacteria and eukaryotes

ABSTRACT: The study of DNA extraction kits

PROVIDER: PRJNA474064 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7268510.fastq.gz	Fastqsanger.gz
	SRR7268520.fastq.gz	Fastqsanger.gz
	SRR7268530.fastq.gz	Fastqsanger.gz
	SRR7268511.fastq.gz	Fastqsanger.gz
	SRR7268521.fastq.gz	Fastqsanger.gz

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Project description:Serum miRNAs are considered useful as non-invasive biomarkers for various diseases, but the optimal method for extracting RNA from serum is currently unknown. In this study, several RNA extraction kits were used to determine which kit is the optimal method. RNA was extracted from the serum of 8-week-old C57BL/6NJcl male mice according to the protocol of each RNA extraction kit. The yield of extracted RNA samples was calculated and electrophoretic patterns were evaluated by Agilent bioanalyzer. Expression patterns of the extracted RNA samples were confirmed by Agilent mouse miRNA microarray. The results showed significant differences in RNA yields in the miRNeasy serum/plasma advanced kit, and mirVana™ PARIS™ RNA and Native Protein Purification Kit compared to almost all other samples. Furthermore, two peaks were identified in the miRNeasy serum/plasma advanced kit using small RNA kit of Agilent bioanalyzer, one at 20-40 nucleotides (nt) and the other around 40-100 nt whereas the other reagents had a single peak. In addition, a high correlation was observed between the two RNA extraction kits in microarray. These results suggest that the above two kits are suitable for miRNA extraction from mouse serum.

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Nassar2022 - Microbome nucleic acid extraction kit NER model

Project description:Microbiome nucleic acid extraction kit model is a Named Entity Recognition (NER) model that identifies and annotates the name of the kits used in extracting microbiome nucleic acids in texts. This is the final model version used to annotate metagenomics publications in Europe PMC and enrich metagenomics studies in MGnify with kits metadata from literature. For more information, please refer to the following blogs: http://blog.europepmc.org/2020/11/europe-pmc-publications-metagenomics-annotations.html https://www.ebi.ac.uk/about/news/service-news/enriched-metadata-fields-mgnify-based-text-mining-associated-publications

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Navigating the hydroxymethylome: experimental biases and quality control tools for the tandem bisulfite and oxidative bisulfite Illumina microarrays

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