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A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma

ABSTRACT: We compared 3 small RNA library prep kits (CleanTag, NEXTflex, QIAseq) and two RNA extraction methods (miRNeasy and MagnaZol) on plasma. We report that library preparation has a significant effect upon the miRNA profile detected, with QIAseq libraries exhibiting the least sequencing bias of the three library kits. RNA extraction methods also contribute, to a lesser extent, to the miRNA profile detected, with MagnaZol RNA extraction increasing the percentage of reads mapping to miRNAs and the number of individual miRNAs detected.

ORGANISM(S): Homo sapiens

PROVIDER: GSE118125 | GEO | 2019/05/21

REPOSITORIES: GEO

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