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Global effects of SUPT4H1 RNAi on gene expression of primary human fibroblasts

ABSTRACT: Global effects of SUPT4H1 RNAi on gene expression of primary human fibroblasts

PROVIDER: PRJNA477932 | ENA |

REPOSITORIES: ENA

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Global effects of SUPT4H1 RNAi on gene expression of primary human fibroblasts

Project description:SUPT4H1 is a transcription elongation factor comprising part of the RNA polymerase II complex. Recent studies propose a selective role for SUPT4H1 in transcription of repeat-containing transcripts; the translated products of which being a hallmark of neurodegeneration in disorders such as Huntington’s Disease and C9orf72-amyotrophic lateral sclerosis. To investigate the potential of SUPT4H1 as a therapeutic target in repeat-associated neurodegeneration, we depleted SUPT4H1 by RNA interference in order to mimic pharmacological loss of function. Using RNA sequencing we assessed SUPT4H1-knockdown induced changes is both immortalized cells (HEK293t) as well as primary human fibroblasts. Diminished SUPT4H1 leads to a global reduction in cellular RNA.

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