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Large-scale tethered function assays identify factors that regulate mRNA stability and translation (HEK293T_RNA-seq)

ABSTRACT: Large-scale tethered function assays identify factors that regulate mRNA stability and translation (HEK293T_RNA-seq)

PROVIDER: PRJNA482136 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7547760.fastq.gz	Fastqsanger.gz
	SRR7547770.fastq.gz	Fastqsanger.gz
	SRR7547780.fastq.gz	Fastqsanger.gz
	SRR7547790.fastq.gz	Fastqsanger.gz
	SRR7547800.fastq.gz	Fastqsanger.gz

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Large-scale tethered function assays identify factors that regulate mRNA stability and translation

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Large-scale tethered function assays identify factors that regulate mRNA stability and translation

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Large-scale tethered function assays identify factors that regulate mRNA stability and translation (HEK293T_RNA-seq)

Project description:we either depleted RBPs by lentiviral transduction of short-hairpin RNAs (shRNAs) or ectopically expressed the open reading frame of the protein. For each RBP, we performed two independent transductions of two different targeting shRNAs and two independent transductions of a non-targeting shRNA, or performed two independent transfections with a plasmid directing expression of the RBP as a V5-tagged fusion, with the FLAG construct as a control.

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