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Genome-wide transcriptional and functional analysis of human T lymphocytes treated with benzo[a]pyrene

ABSTRACT: Genome-wide transcriptional and functional analysis of human T lymphocytes treated with benzo[a]pyrene

PROVIDER: PRJNA482396 | ENA |

REPOSITORIES: ENA

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Genome-wide transcriptional and functional analysis of human T lymphocytes treated with benzo[a]pyrene

Project description:Polycyclic aromatic hydrocarbons (PAHs) such as benzo[a]pyrene (B[a]P), are widely distributed carcinogenic and immunotoxic environmental contaminants, known to affect T lymphocytes. Despite extensive studies conducted, the molecular targets and pathways involved in their immunotoxic effects in human T lymphocytes remain unknown. Here, we analyzed the gene expression profile of primary human T lymphocytes treated with the prototypical PAH, B[a]P using a microarray-based transcriptome analysis. After a 48-h exposure to B[a]P, we identified 158 genes differentially expressed in T lymphocytes including not only genes well-known to be affected by PAHs such as the cytochromes P450 (CYP) 1A1 and 1B1, and the proto-oncogene c-KIT but also other ones not previously shown to be targeted by B[a]P such as genes encoding the gap junction beta-2 (GJB2) and beta-6 (GJB6) proteins. Functional enrichment analysis revealed that those candidates were significantly associated with the aryl hydrocarbon (AhR) and interferon (IFN) signalling pathways; it also revealed a clear alteration in essential biological functions, mainly those related to T lymphocyte recruitment. These data were confirmed by RT-qPCR assays for some selected genes such as the chemokine (C-C motif) ligand CCL3, and the cell adhesion protein like selectin L (SELL). Using functional tests in transwell migration experiments, B[a]P was shown to significantly decrease the chemokine (C-X-C motif) ligand 12 (CXCL12)-induced chemotaxis and transendothelial migration of T lymphocytes. Overall, the present study opens the way to unsuspected responsive pathway of interest, i.e., T lymphocyte migration, providing a broader understanding of the molecular basis of the immunotoxicity of PAHs.

2018-12-26 | GSE117527 | GEO

Genome-wide transcriptional and functional analysis of human T lymphocytes treated with benzo[a]pyrene

Project description:Genome-wide transcriptional and functional analysis of human T lymphocytes treated with benzo[a]pyrene

| PRJNA482396 | ENA

Cytochrome P450 Monooxygenase Mediated Metabolic Utilization of Benzo(a)pyrene by Fungi

Project description:Soil dwelling Aspergillus fungi possess the versatile metabolic capability to utilize complex organic compounds which are toxic to humans, yet the mechanisms they employ remain largely unknown. Benzo(a)pyrene is a common carcinogenic contaminant, posing a significant concern for human health. Here, we report that Aspergillus fungi can degrade benzo(a)pyrene effectively. In Aspergillus nidulans, exposure to benzo(a)pyrene results in transcriptomic and metabolic changes associated with cellular growth and energy generation, implying that the fungus utilizes benzo(a)pyrene as a food. Importantly, we identify and characterize the conserved bapA gene encoding a cytochrome P450 monooxygenase that exerts the first step in the degradation of benzo(a)pyrene. We further demonstrate that the fungal NF-κB-type global regulators VeA and VelB are required for benzo(a)pyrene degradation in A. nidulans, which occurs through expression control of bapA in response to nutrient limitation. Our study illuminates fundamental knowledge of fungal benzo(a)pyrene metabolism and provides novel insights into enhancing bioremediation potential.

2018-07-10 | GSE116804 | GEO

Glutathione S-transferase induction after exposure to benzo[a]pyrene

Project description:Benzo[a]pyrene induction of glutathione S-transferases, an activity-based protein profiling investigation

2020-05-26 | PXD010610 | Pride

Acute hepatic transcriptional response in male mice following oral exposure to benzo(a)pyrene

Project description:Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male B6C3F1 mice were exposed to four doses of benzo(a)pyrene or vehicle control for three days and sacrificed 4 or 24 hours after the final exposure.

2015-07-01 | GSE43633 | GEO

Modulating the Response to Benzo[a]pyrene when CYP1A1 and CYP1B1 are Knocked Down

Project description:Benzo[a]pyrene is a Group 1 carcinogen. It undergoes metabolism in the liver through CYP1A1 and CYP1B1 enzymes. This study was conducted to investigate the response to benzo[a]pyrene in the liver when these enzymes are knocked down.

2024-01-03 | GSE252339 | GEO

Sub-chronic forestomach transcriptional response in adult male MutaTMMouse following oral exposure to benzo(a)pyrene

Project description:Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male MutaTMMouse were exposed to three doses of benzo(a)pyrene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure.

2013-09-27 | GSE43438 | GEO

Effect of the genotoxic human lung carcinogen benzo[a]pyrene and its non-carcinogenic isomer benzo[e]pyrene on transcriptomic profiles in HepaRG cells.

Project description:Twenty-eight days after the initial seeding, the terminally differentiated HepaRG cells were treated with 2 µM benzo[a]pyrene (B[a]P) and benzo[e]pyrene (B[e]P) for 72 hours. Following the treatment, the cells were harvested by mild trypsinization, washed in phosphate-buffered saline, and immediately frozen at −80°C for subsequent analyses. Gene expression profiles in the HepaRG cells treated with B[a]P and B[e]P were investigated using Agilent whole genome 8x60K human microarrays according to the manufacturer’s instructions.

2018-07-24 | GSE117476 | GEO

Transcriptomic response to benzo[a]pyrene treatment in HepG2 cells

Project description:This SuperSeries is composed of the following subset Series: GSE36242: Transcriptomic response to benzo[a]pyrene treatment in HepG2 cells (RNA-Seq) GSE36243: Transcriptomic response to benzo[a]pyrene treatment in HepG2 cells (Affymetrix) Refer to individual Series

2012-11-16 | E-GEOD-36244 | biostudies-arrayexpress

Microarray data of benzo[a]pyrene-treated WHCO1 vs EPC-2 cells

Project description:The aim of this project was to investigate the differential effects of benzo[a]pyrene (BaP) treatment on global gene transcription, comparing normal (EPC-2) and cancer (WHCO1) cell lines from the oesophagus, in order to further our understanding of the molecular mechanisms of BaP-induced development and maintenance of oesophageal squamous cell carcinoma.

2018-01-18 | GSE109316 | GEO

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