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Mirounga angustirostris

ABSTRACT: Mirounga angustirostris blubber transcriptome response to repeated ACTH administration

PROVIDER: PRJNA485363 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7668300_1.fastq.gz	Fastqsanger.gz
	SRR7668300_2.fastq.gz	Fastqsanger.gz
	SRR7668301_1.fastq.gz	Fastqsanger.gz
	SRR7668301_2.fastq.gz	Fastqsanger.gz
	SRR7668302_1.fastq.gz	Fastqsanger.gz

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Molecular Population Genetics of the Northern Elephant Seal Mirounga angustirostris.

Project description:The northern elephant seal, Mirounga angustirostris, was heavily hunted and declared extinct in the 19th century. However, a colony remained on remote Guadalupe Island, Mexico and the species has since repopulated most of its historical distribution. Here, we present a comprehensive evaluation of genetic variation in the species. First, we assess the effect of the demographic bottleneck on microsatellite variability and compare it with that found in other pinnipeds, demonstrating levels of variation similar to that in species that continue to be threatened with extinction. Next, we use sequence data from these markers to demonstrate that some of the limited polymorphism predates the bottleneck. However, most contemporary variation appears to have arisen recently and persisted due to exponential growth. We also describe how we use the range in allele size of microsatellites to estimate ancestral effective population size before the bottleneck, demonstrating a large reduction in effective size. We then employ a classical method for bacteria to estimate the microsatellite mutation rate in the species, deriving an estimate that is extremely similar to that estimated for a similar set of loci in humans, indicating consistency of microsatellite mutation rates in mammals. Finally, we find slight significant structure between some geographically separated colonies, although its biological significance is unclear. This work demonstrates that genetic analysis can be useful for evaluating the population biology of the northern elephant seal, in spite of the bottleneck that removed most genetic variation from the species.

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Campylobacter insulaenigrae isolates from northern elephant seals (Mirounga angustirostris) in California.

Project description:There are only two reports in the literature demonstrating the presence of Campylobacter spp. in marine mammals. One report describes the isolation of a new species, Campylobacter insulaenigrae sp. nov., from three harbor seals (Phoca vitulina) and a harbor porpoise (Phocoena phocoena) in Scotland, and the other describes the isolation of Campylobacter jejuni, Campylobacter lari, and an unknown Campylobacter species from northern elephant seals (Mirounga angustirostris) in California. In this study, 72 presumptive C. lari and unknown Campylobacter species strains were characterized using standard phenotypic methods, 16S rRNA PCR, and multilocus sequence typing (MLST). Phenotypic characterization of these isolates showed them to be variable in their ability to grow either at 42 degrees C or on agar containing 1% glycine and in their sensitivity to nalidixic acid and cephalothin. Based on both 16S rRNA PCR and MLST, all but 1 of the 72 isolates were C. insulaenigrae, with one isolate being similar to but distinct from both Campylobacter upsaliensis and Campylobacter helveticus. Phylogenetic analysis identified two C. insulaenigrae clades: the primary clade, containing exclusively California strains, and a secondary clade, containing some California strains and all of the original Scottish strains. This study demonstrates the inability of phenotypic characterization to correctly identify all Campylobacter species and emphasizes the importance of molecular characterization via 16S rRNA sequence analysis or MLST for the identification of Campylobacter isolates from marine mammals.

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Blubber proteome response to repeated ACTH administration in juvenile elephant seals

Project description:Proteins were isolated from the inner blubber layer (closest to muscle) of juvenile elephant seals undergoing a repeated ACTH administration experiment (for details, see McCormley et al., 2018, doi: 10.1093/conphys/coy040; Deyarmin et al., 2019, doi:10.1038/s41598-019-39089-2). Sample preparation was conducted using the Qiazol method as previously described (Khudyakov et al., 2018, doi:10.1242/bio.036731). Four samples of blubber proteins from each of 2 seals (collected before and after the first and fourth ACTH administration) were labeled with 8-plex iTRAQ, fractionated off-line, pooled into 4 fractions, and analyzed by HPLC-MS/MS.

2019-07-29 | MSV000084153 | MassIVE

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