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Defective mitochondrial cardiolipin remodeling dampens HIF-1a expression in hypoxia

ABSTRACT: Defective mitochondrial cardiolipin remodeling dampens HIF-1a expression in hypoxia

PROVIDER: PRJNA490270 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7817300.fastq.gz	Fastqsanger.gz
	SRR7817301.fastq.gz	Fastqsanger.gz
	SRR7817302.fastq.gz	Fastqsanger.gz
	SRR7817303.fastq.gz	Fastqsanger.gz
	SRR7817294.fastq.gz	Fastqsanger.gz

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Defective mitochondrial cardiolipin remodeling dampens HIF-1a expression in hypoxia

Project description:Mitochondria fulfill vital metabolic functions and act as crucial cellular signaling hubs integrating their metabolic status into the cellular context. Here, we show that defective cardiolipin-remodeling, upon loss of the cardiolipin acyl transferase Tafazzin, mutes HIF-1a signaling in hypoxia. Tafazzin-deficiency does not affect posttranslational HIF-1a regulation but rather HIF-1a gene-expression, a dysfunction recapitulated in iPSCs-derived cardiomyocytes from Barth Syndrome patients with Tafazzin-deficiency. RNAseq analyses confirmed drastically altered signaling in Tafazzin mutant cells. In hypoxia, Tafazzin-deficient cells display reduced production of reactive oxygen species (ROS) perturbing NF-kB activation and concomitantly HIF-1a gene-expression. In agreement, Tafazzin-deficient mice hearts display reduced HIF-1a levels and undergo maladaptive hypertrophy with heart failure in response to pressure overload challenge. We conclude that defective mitochondrial cardiolipin-remodeling dampens HIF-1a signaling through inactivation of a non-canonical signaling pathway: Lack of NF-kB activation through reduced mitochondrial ROS production diminishes HIF-1a transcription.

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Defective mitochondrial cardiolipin remodeling dampens HIF-1a expression in hypoxia

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Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.

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