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Targets of Hnf4alpha during conversion from mouse embryonic fibroblasts (MEFs) to hepatocyte-like (iHep) cells.

ABSTRACT: Targets of Hnf4alpha during conversion from mouse embryonic fibroblasts (MEFs) to hepatocyte-like (iHep) cells.

PROVIDER: PRJNA490545 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10901265.fastq.gz	Fastqsanger.gz
	SRR10901266.fastq.gz	Fastqsanger.gz
	SRR7823490.fastq.gz	Fastqsanger.gz
	SRR7823491.fastq.gz	Fastqsanger.gz
	SRR7823492.fastq.gz	Fastqsanger.gz

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Targets of Hnf4alpha during conversion from mouse embryonic fibroblasts (MEFs) to hepatocyte-like (iHep) cells.

Project description:We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used ChIP-seq to explore the targets of Hnf4alpha during conversion event from MEFs to iHep cells.

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Foxa and Hnf4alpha targets during conversion from mouse embryonic fibroblasts (MEFs) to hepatocyte-like (iHep) cells

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Project description:We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used ChIP-seq to explore the targets of Foxa and Hnf4alpha during conversion event to iHep cells. Besides we performed for hepatocytes.

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Project description:We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2, or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes (iHep cells) in vitro. Then we mapped 2 histone modification marks (H3K9ac and H3K27me3) and 2 states of Pol2 (with phosphorylated serine 5 and phosphorylated both of serine 5 and serine 2) during the conversion event. Besides we performed FAIRE-Seq to explore the chromatin accessbility in the iHep cells.

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Project description:We have found that three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro, and DNA binding, N-terminal, and C-terminal domains (DBD, NTD, and CTD, respectively) of Foxa proteins are swappable in the direct reprogramming of the iHep cells. Then we used ChIP-seq to explore the targets of the domain swapped mutant forms of Foxa proteins during conversion event to iHep cells.

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Project description:In order to characterize iHep cells more precisely, we conducted global gene expression analyses using microarrays to compare among the gene expression profiles of MEFs, iHep cells and adult mouse hepatocytes.

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