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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells [RRBS]

ABSTRACT: Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells [RRBS]

PROVIDER: PRJNA491433 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7852010_1.fastq.gz	Fastqsanger.gz
	SRR7852010_2.fastq.gz	Fastqsanger.gz
	SRR7852020_1.fastq.gz	Fastqsanger.gz
	SRR7852020_2.fastq.gz	Fastqsanger.gz
	SRR7852001_1.fastq.gz	Fastqsanger.gz

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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells [RRBS]

Project description:Uniparental reproduction is widespread among lower vertebrates, but not in mammals. Researchers have produced bimaternal mice with a deletion of the H19 imprinted region. However, the mechanism of a single deletion in an immature oocyte sufficient to cross uniparental barriers is unknown. We found bimaternal-derived mice to be defective in multiple aspects. More importantly, bipaternal reproduction has not been achieved in mammals. Thus, the barrier of uniparental reproduction in mammals has not been elucidated. We identified a DNA hypomethylation status in haploid embryonic stem cells similar to that in primordial germ cells, with which bimaternal and bipaternal mice can be obtained by injection of haploid ES cells with genetic modifications. The phenotypic analyses of derived mice support the genetic conflict theory of genomic imprinting. Taken together, our results highlight the factors necessary for crossing uniparental reproduction barriers in mammals.

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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells

Project description:Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells

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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells [RNA-Seq]

Project description:Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells [RNA-Seq]

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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:We generate histone modification profiles and DNA methylation profiles of mouse haploid embryonic stem cells. By comparison to mouse diploid ESCs and MEFs, we found the similar chromatin landscapes between haESCs and mESCs, which reveal the self-renew ability and pluripotency in haESCs. Besides, haESCs specific chromatin landscapes show its sperm-like functions.

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Project description:Nucleosomes are barriers to transcription in vitro, however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA Polymerase II (RNAPII) at nucleotide resolution. Our results suggest that nucleosomes present significant, context-specific barriers to RNAPII in vivo that can be tuned by the incorporation of H2A.Z. 8 sets of two replicates each of paired-end and 3 sets of two replicates each of single-end samples were sequenced and analyzed.

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