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Achilles-mediated and sex-specific regulation of circadian mRNA rhythms in Drosophila

ABSTRACT: Achilles-mediated and sex-specific regulation of circadian mRNA rhythms in Drosophila

PROVIDER: PRJNA491652 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7868200.fastq.gz	Fastqsanger.gz
	SRR7868210.fastq.gz	Fastqsanger.gz
	SRR7868220.fastq.gz	Fastqsanger.gz
	SRR7868230.fastq.gz	Fastqsanger.gz
	SRR7868240.fastq.gz	Fastqsanger.gz

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Achilles-mediated and sex-specific regulation of circadian mRNA rhythms in Drosophila

Project description:The circadian clock is an evolutionarily conserved mechanism that drives rhythmic expression of downstream genes. The core circadian clock drives the expression of clock-controlled genes either directly or indirectly, which in turn play critical roles in carrying out many rhythmic physiological processes. Nevertheless, the molecular mechanisms by which clock output genes orchestrate rhythmic signals from the brain to peripheral tissues are largely unknown. Here we explored the role of one rhythmic gene, Achilles, in regulating the rhythmic transcriptome in fly heads. Achilles is a clock-controlled gene in Drosophila that encodes a putative RNA-binding protein. Achilles expression is not detectable in core clock neurons using in-situ hybridization, although its expression is found in neurons throughout the fly brain. Together, these observations argue against a role for Achilles in regulating the core clock. To assess its impact on circadian mRNA rhythms, we performed RNA sequencing (RNAseq) to compare the rhythmic transcriptomes of control flies and those with diminished Achilles expression in all neurons. Consistent with previous observations, we observe dramatic upregulation of immune response genes upon knock-down of Achilles. Furthermore, a subset of circadian mRNAs lose their rhythmicity in Achilles knock-down flies, suggesting that a subset of the rhythmic transcriptome is regulated either directly or indirectly by Achilles. These Achilles-mediated rhythms include many genes involved in immune function and neuronal signaling such as Prosap, Nemy and Jhl-21. Comparison of RNAseq data from control flies reveal that only 42.7% of clock-controlled genes in the fly brain are rhythmic in both males and females. As mRNA rhythms of core clock genes are largely invariant between the sexes, this observation suggests that sex-specific mechanisms are an important, and heretofore under-appreciated, regulator of the rhythmic transcriptome.

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