Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells

ABSTRACT: Molecular and functional heterogeneity of IL-10-producing CD4+ T cells

PROVIDER: PRJNA497253 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8060980.fastq.gz	Fastqsanger.gz
	SRR8060981.fastq.gz	Fastqsanger.gz
	SRR8060842_1.fastq.gz	Fastqsanger.gz
	SRR8060842_2.fastq.gz	Fastqsanger.gz
	SRR8060843_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 780

Similar Datasets

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse 10x]

Project description:IL-10 is a prototypical anti-inflammatory cytokine, which is fundamental to the maintenance of immune homeostasis, especially in the intestine. There is an assumption that cells producing IL-10 have an immunoregulatory function. However, here we report that IL-10-producing CD4+ T cells are phenotypically and functionally heterogeneous. By combining single cell transcriptome and functional analyses, we identified a subpopulation of IL-10-producing Foxp3Neg CD4+ T cells that displays regulatory activity unlike other IL-10-producing CD4+ T cells, which are unexpectedly pro-inflammatory. The combinatorial expression of co-inhibitory receptors is sufficient to discriminate IL-10-producing CD4+ T cells with regulatory function from others and to identify them across different tissues and disease models in mice and humans. These regulatory IL-10-producing Foxp3Neg CD4+ T cells have a unique transcriptional program, which goes beyond the regulation of IL-10 expression. Finally, we found that patients with Inflammatory Bowel Disease (IBD), demonstrate a deficiency in this specific regulatory T-cell subpopulation.

2018-11-07 | GSE121287 | GEO

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Human 10x]

2018-11-07 | GSE121267 | GEO

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

2018-11-07 | GSE121386 | GEO

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse Bulk-seq]

2018-11-07 | GSE121284 | GEO

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse 10x]

Project description:Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse 10x]

| PRJNA496523 | ENA

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Human 10x]

Project description:Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Human 10x]

| PRJNA496578 | ENA

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

Project description:Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

| PRJNA497250 | ENA

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse Bulk-seq]

Project description:Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse Bulk-seq]

| PRJNA496521 | ENA

Single cell RNA-seq of IL-10-producing CD4 T cells during chronic LCMV infection

Project description:During chronic viral infection, the inflammatory function of CD4 T cells becomes gradually attenuated. Concurrently, Th1 cells progressively acquire the capacity to secrete the cytokine IL-10, a potent suppressor of antiviral T cell responses. To determine the transcriptional changes that underlie this T cell adaption process, we applied a single-cell RNA-sequencing approach and assessed the heterogeneity of IL-10-expressing CD4 T cells during chronic infection. Unexpectedly, our analyses revealed an IL-10-producing population with a robust Tfh-signature. Using IL-10 and IL-21 double-reporter mice, we further demonstrate that IL-10+IL-21+co-producing Tfh cells arise predominantly during chronic but not acute LCMV infection. Importantly, depletion of IL-10+IL-21+co-producing CD4 T cells or deletion of Il10 specifically in Tfh cells resulted in impaired humoral immunity and viral control. Mechanistically, B cell-intrinsic IL-10 signaling was required for sustaining germinal center reactions. Lastly, we demonstrate that IL-27 and type I IFNs differentially regulate the formation of this protective IL-10-producing Tfh subset. Thus, our findings elucidate a critical role for Tfh-derived IL-10 in promoting humoral immunity during persistent viral infection.

2018-10-29 | GSE111026 | GEO

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells.

Project description:IL-10 is a prototypical anti-inflammatory cytokine, which is fundamental to the maintenance of immune homeostasis, especially in the intestine. There is an assumption that cells producing IL-10 have an immunoregulatory function. However, here we report that IL-10-producing CD4+ T cells are phenotypically and functionally heterogeneous. By combining single cell transcriptome and functional analyses, we identified a subpopulation of IL-10-producing Foxp3neg CD4+ T cells that displays regulatory activity unlike other IL-10-producing CD4+ T cells, which are unexpectedly pro-inflammatory. The combinatorial expression of co-inhibitory receptors is sufficient to discriminate IL-10-producing CD4+ T cells with regulatory function from others and to identify them across different tissues and disease models in mice and humans. These regulatory IL-10-producing Foxp3neg CD4+ T cells have a unique transcriptional program, which goes beyond the regulation of IL-10 expression. Finally, we found that patients with Inflammatory Bowel Disease demonstrate a deficiency in this specific regulatory T-cell subpopulation.

| S-EPMC6303294 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data