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Single-cell RNA-seq of Drosophila melanogaster male and female late third instar larval gonads

ABSTRACT: Single-cell RNA-seq of Drosophila melanogaster male and female late third instar larval gonads

PROVIDER: PRJNA518094 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8513910.fastq.gz	Fastqsanger.gz
	SRR8513911.fastq.gz	Fastqsanger.gz
	SRR8513912.fastq.gz	Fastqsanger.gz
	SRR8513906.fastq.gz	Fastqsanger.gz
	SRR8513907.fastq.gz	Fastqsanger.gz

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Drosophila melanogaster female late third instar larval gonads single-cell RNA-Seq (scRNA-Seq) profiling

Project description:The larval ovary is made up of multiple cell types including germ cells and somatic cells. The diversity of cell types and transcriptional regulation is not fully understood. To get single cell resolution of larval ovary regulation, we generated single-cell RNA expression profiles (scRNA-Seq) from late third instar larval ovaries of a reference Drosophila melanogaster genotype w[1118].

2019-02-01 | GSE125948 | GEO

Single-cell RNA-seq of Drosophila melanogaster male late third instar larval gonads

Project description:Single-cell RNA-seq of Drosophila melanogaster male late third instar larval gonads

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Single-cell RNA-seq of Drosophila melanogaster male late third instar larval gonads

Project description:The male larval gonad is a complex tissue made up of multiple cell types including germ cells and somatic cells. It is also a key developmental stage with the germline undergoing spermatogenesis. The diversity of cell types and transcriptional regulation of the developing gonad has only been broadly described. In order to get single cell resolution, we profiled late third instar larval testes of a reference Drosophila melanogaster genotype, w[1118] using single-cell RNA-Seq (scRNA-Seq). We identified and annotated 9 cell types including 4 stages of the germ cell lineage, and provide transcriptional profiles for each cell type.

2019-02-01 | GSE125947 | GEO

Drosophila melanogaster female late third instar larval gonads single-cell RNA-Seq (scRNA-Seq) profiling

Project description:Drosophila melanogaster female late third instar larval gonads single-cell RNA-Seq (scRNA-Seq) profiling

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Single-cell RNA-seq of Drosophila melanogaster male and female late third instar larval gonads

Project description:Single-cell RNA-seq of Drosophila melanogaster male and female late third instar larval gonads

| PRJNA518094 | ENA

Expression profile of third instar larval fat body and midgut tissues

Project description:We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male larvae and gonads were removed to eliminate contaminating transctips from the gonads. Larval midguts were dissected from ~50 wandering third instar larvae. Larval tissues were removed to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 5ug of total RNA and subject to Illumina based sequencing.

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Drosophila melanogaster late third instar larval ovary and gonadal fatbody polyA+ RNA-Seq profiling.

Project description:Here we perform transcriptional profiling of late third instar larval ovaries and gonadal fatbody from Drosophila melanogaster w1118 flies. We designed this experiment to investigate the effect of protease treatment on dissected ovaries. We profiled ovaries without protease treatment with some fatbody still attached, fatbody alone dissected from around the ovaries, ovaries cleaned of fatbody using a papain/collagenase cocktail, and ovaries dissociated using a papain/collagenase cocktail. We then constructed polyA+ libraries and performed 50 bp stranded single end RNA-Seq.

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Drosophila melanogaster late third instar larval testes and gonadal fatbody RNA-seq profiling

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Project description:We designed this experiment to investigate the transcriptional changes in gonads as a result of sex transformation. Here we performed transcriptional profiling of the ovary transformed into testis from the tra loss of function (XX_tra_lof), testis transformed into ovary from the tra gain of function (XY_tra_gof) and ovary transformed into testis in dsxM gain of function (XX_DsxM_gof/lof) Drosophila melanogaster third instar larvae in biological quadruplicates. In addition, as controls we sequenced ovaries and testes from the female and male wildtype larvae respectively. We constructed polyA+ libraries of the gonads, cleaned off the fatbody and performed 50 bp, stranded single-end RNA-Seq.

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