Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression profile of third instar larval fat body and midgut tissues

ABSTRACT: We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male larvae and gonads were removed to eliminate contaminating transctips from the gonads. Larval midguts were dissected from ~50 wandering third instar larvae. Larval tissues were removed to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 5ug of total RNA and subject to Illumina based sequencing.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Terry Orr-Weaver 

PROVIDER: E-GEOD-25025 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Developmental control of the DNA replication and transcription programs.

Nordman Jared J   Li Sharon S   Eng Thomas T   Macalpine David D   Orr-Weaver Terry L TL  

Genome research 20101222 2

Polyploid or polytene cells, which have more than 2C DNA content, are widespread throughout nature and present in most differentiated Drosophila tissues. These cells also can display differential replication, that is, genomic regions of increased or decreased DNA copy number relative to overall genomic ploidy. How frequently differential replication is used as a developmental strategy remains unclear. Here, we use genome-wide array-based comparative genomic hybridization (aCGH) to profile differ  ...[more]

Similar Datasets

Transcriptomics Expression profile of third instar larval salivary gland tissue
2011-10-18 | E-GEOD-33017 | biostudies-arrayexpress
Genomics ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing
2011-10-18 | E-GEOD-31899 | biostudies-arrayexpress
Transcriptomics Developmental Control of Gene Copy Number by Repression of Replication Initiation and Fork Progression
2011-10-17 | E-GEOD-31900 | biostudies-arrayexpress
Genomics Identification of lineage-specific cis-regulatory modules associated with variation in transcription factor binding and chromatin activity using Ornstein-Uhlenbeck models
2015-06-17 | GSE59706 | GEO
Transcriptomics Dynamics and stage-specificity of between-population gene expression divergence in the Drosophila melanogaster larval fat body (RNA-seq in a derived population)
2023-04-04 | GSE218763 | GEO
Transcriptomics Expression data from 0.15M and 0.7M-fed wild-type and ChREBP mutant, third instar Drosophila larval fat bodies (FBs)
2013-01-25 | E-GEOD-43734 | biostudies-arrayexpress
Transcriptomics Identification of 20E-regulated genes in Drosophila cultured larval organs
2005-10-04 | GSE3060 | GEO
Genomics ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing
2011-10-18 | GSE31899 | GEO
Proteomics proteomics in Drosophila fat body
2021-11-25 | PXD029921 |
Transcriptomics Single-cell RNA-seq on Drosophila melanogaster wild type third instar eye discs
2018-10-29 | E-MTAB-7195 | biostudies-arrayexpress