Unknown,Transcriptomics,Genomics,Proteomics

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Expression profile of third instar larval fat body and midgut tissues

ABSTRACT: We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male larvae and gonads were removed to eliminate contaminating transctips from the gonads. Larval midguts were dissected from ~50 wandering third instar larvae. Larval tissues were removed to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 5ug of total RNA and subject to Illumina based sequencing.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Terry Orr-Weaver 

PROVIDER: E-GEOD-25025 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Developmental control of the DNA replication and transcription programs.

Nordman Jared J   Li Sharon S   Eng Thomas T   Macalpine David D   Orr-Weaver Terry L TL  

Genome research 20101222 2

Polyploid or polytene cells, which have more than 2C DNA content, are widespread throughout nature and present in most differentiated Drosophila tissues. These cells also can display differential replication, that is, genomic regions of increased or decreased DNA copy number relative to overall genomic ploidy. How frequently differential replication is used as a developmental strategy remains unclear. Here, we use genome-wide array-based comparative genomic hybridization (aCGH) to profile differ  ...[more]

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