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Functional Cardiac Fibroblasts Derived from Human Pluripotent Stem Cells via Second Heart Field Progenitors

ABSTRACT: Functional Cardiac Fibroblasts Derived from Human Pluripotent Stem Cells via Second Heart Field Progenitors

PROVIDER: PRJNA521412 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8548580.fastq.gz	Fastqsanger.gz
	SRR8548571.fastq.gz	Fastqsanger.gz
	SRR8548572.fastq.gz	Fastqsanger.gz
	SRR8548573.fastq.gz	Fastqsanger.gz
	SRR8548574.fastq.gz	Fastqsanger.gz

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Similar Datasets

Functional Cardiac Fibroblasts Derived from Human Pluripotent Stem Cells via Second Heart Field Progenitors

Project description:Cardiac fibroblasts (CFs) play critical roles in heart development, homeostasis, and disease. The limited availability of human CFs from native heart impedes investigations of CF biology and their role in disease. Human pluripotent stem cells (hPSCs) provide a highly renewable and genetically defined cell source, but efficient methods to generate CFs from hPSCs have not been described. Here, we show differentiation of hPSCs using sequential modulation of Wnt and FGF signaling to generate second heart field progenitors that efficiently give rise to hPSC-CFs. The hPSC-CFs resemble native heart CFs in cell morphology, proliferation, gene expression, fibroblast marker expression, production of extracellular matrix and myofibroblast transformation induced by TGFβ1 and angiotensin II. Furthermore, hPSC-CFs exhibit a more embryonic phenotype when compared to fetal and adult primary human CFs. Co-culture of hPSC-CFs with hPSC-derived cardiomyocytes distinctly alters the electrophysiological properties (EP) of the cardiomyocytes compared to co-culture with dermal fibroblasts (DFs). The hPSC-CFs provide a powerful cell source for research, drug discovery, precision medicine, and therapeutic applications in cardiac regeneration.

2019-02-08 | GSE126260 | GEO

Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors.

Project description:Cardiac fibroblasts (CFs) play critical roles in heart development, homeostasis, and disease. The limited availability of human CFs from native heart impedes investigations of CF biology and their role in disease. Human pluripotent stem cells (hPSCs) provide a highly renewable and genetically defined cell source, but efficient methods to generate CFs from hPSCs have not been described. Here, we show differentiation of hPSCs using sequential modulation of Wnt and FGF signaling to generate second heart field progenitors that efficiently give rise to hPSC-CFs. The hPSC-CFs resemble native heart CFs in cell morphology, proliferation, gene expression, fibroblast marker expression, production of extracellular matrix and myofibroblast transformation induced by TGF?1 and angiotensin II. Furthermore, hPSC-CFs exhibit a more embryonic phenotype when compared to fetal and adult primary human CFs. Co-culture of hPSC-CFs with hPSC-derived cardiomyocytes distinctly alters the electrophysiological properties of the cardiomyocytes compared to co-culture with dermal fibroblasts. The hPSC-CFs provide a powerful cell source for research, drug discovery, precision medicine, and therapeutic applications in cardiac regeneration.

| S-EPMC6527555 | biostudies-literature

Subpopulations second heart field RNA-seq

Project description:Transcriptomic profiling of second heart field subpopulations FACS sorted

2020-08-31 | GSE123771 | GEO

Subpopulations second heart field ATAC-seq

Project description:Profiling of open chromatin on second heart field subpopulations FACS sorted

2020-08-18 | GSE123765 | GEO

Subpopulations second heart field RNA-seq

Project description:Subpopulations second heart field RNA-seq

| PRJNA509773 | ENA

Subpopulations second heart field ATAC-seq

Project description:Subpopulations second heart field ATAC-seq

| PRJNA509774 | ENA

Hedgehog Molecular Networks in the Second Heart Field

Project description:Hedgehog Molecular Networks in the Second Heart Field

| PRJNA191539 | ENA

A Second Heart Field-derived vasculogenic niche contributes to cardiac lymphatics

Project description:A Second Heart Field-derived vasculogenic niche contributes to cardiac lymphatics

| PRJNA563574 | ENA

A Second Heart Field-derived vasculogenic niche contributes to cardiac lymphatics

Project description:The mammalian heart contains multiple cell types that appear progressively during embryonic development. Advances in determining cardiac lineage diversification has often been limited by the unreliability of genetic tracers. Here we combine clonal analysis, genetic lineage tracing, tissue transplantation and mutant characterization to investigate the lineage relationships between epicardium, arterial mesothelial cells (AMCs) and the coronary vasculature. We report a contribution of the second heart field (SHF) to a vasculogenic niche composed of AMCs and sub-mesothelial cells at the base of the pulmonary artery. Sub-mesothelial cells from this niche differentiate into lymphatic endothelial cells and, in close association with AMC-derived cells, contribute to, and are essential for the development of ventral cardiac lymphatics. In addition, regionalized epicardial/mesothelial retinoic acid signaling regulates lymphangiogenesis, contributing to the niche properties. These results uncover a SHF vasculogenic contribution to coronary lymphatic development through a local niche at the base of the great arteries.

2020-01-09 | GSE136750 | GEO

Heart fields auto-regulate second heart field cell fate via Wnt secretion

Project description:The purpose of this study was to identify the source of Wnt signals that lead to development/proliferation of the heart fields, particularly the second heart field. To this end, we knocked out Wntless (Wls), which is necessary for Wnt secretion, in Mesp1+ lineage cells. We performed single cell RNA-seq to better identify the source of Wnt signals and the effects of the loss of Wnt secretion in mesoderm.

2021-01-23 | GSE165300 | GEO

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