Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Colistin resistance emerges with pandrug-resistance in epidemic clones in Klebsiella pneumoniae in Rio de Janeiro, Brazil

PROVIDER: PRJNA521546 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SSBW01.dat.gz	Other
	SSBW01.fasta.gz	Fasta.gz
	SSBW01.master.dat	Other
	SSCC01.dat.gz	Other

Items per page:

1 - 5 of 10

Similar Datasets

Project description:SARS-CoV-2 genome sequencing Rio de Janeiro / Brazil

| PRJNA686081 | ENA

Project description:Rio de Janeiro Coastline

| PRJEB15044 | ENA

Project description:Virome in roof-harvested rainwater in Rio de Janeiro, Brazil

| PRJNA767339 | ENA

Transcription profiling by array of human bladder cancer cell lines with or without TP53 mutations

Project description:The established cell lines RT4, 5637 and T24 from human bladder TCC were obtained from the Cell Bank of the Federal University of Rio de Janeiro, Brazil. The 5637 cells harbor two TP53 mutations, at codon 72 (Arg . Pro) and codon 280 (Arg . Thr).T24 cells contain a TP53 allele encoding an in-frame deletion of tyrosine 126. Both cell lines were established from high-grade bladder tumors. No specific mutations were detected in RT4 cells, which had been established from a low-grade papillary bladder tumor. The RT4 and T24 cell lines were maintained in DulbeccoM-^Rs modified EagleM-^Rs medium (Sigma-Aldrich, Inc, St Louis, MO, USA), and the 5637 cells were kept in Roswell Park Memorial Institute medium (Sigma-Aldrich). Both media were supplemented with 10% fetal bovine serum (Cultilab Ltd, Campinas, Brazil), 100 U/mL penicillin G (Sigma-Aldrich), 100 U/mL streptomycin (Sigma-Aldrich) and 1% kanamycin sulfate (Amresco, Branded Products Group, Solon, OH, USA); cells were cultured at 37M-:C in an atmosphere of 5% CO2. A pooled reference design was chosen. More specifically, each array was hybridized with the same reference sample labeled with Cy5, while the experimental samples (control or treated) were labeled with Cy3.

2010-10-30 | E-MEXP-2859 | biostudies-arrayexpress

Project description:Ultra-small bacterial community from Jacarepagua Lagoon, Rio de Janeiro, Brazil

| PRJNA629884 | ENA

Proteomic changes of Klebsiella pneumoniae in response to colistin treatment and crrB mutation-mediated colistin resistance

Project description:Polymyxins are increasingly used as the critical last-resort therapeutic options for multidrug-resistant gram-negative bacteria. Unfortunately, polymyxin resistance has increased gradually for the last few years. Although studies on mechanisms of polymyxin are expanding, system-wide analyses of the underlying mechanism for polymyxin resistance and stress response are still lacking. To understand how Klebsiella pneumoniae adapt to colistin (polymyxin E) pressure, we carried out proteomic analysis of Klebsiella pneumoniae strain cultured with different concentrations of colistin. Our results showed that the proteomic responses to colistin treatment in Klebsiella pneumoniae involving several pathways, including (i) gluconeogenesis and TCA cycle; (ii) arginine biosynthesis; (iii) porphyrin and chlorophyll metabolism; and (iv) enterobactin biosynthesis. Interestingly, decreased abundance of class A β-lactamases including TEM, SHV-11, SHV-4 were observed in cells treated with colistin. Moreover, we also present comprehensive proteome atlases of paired polymyxin-susceptible and -resistant Klebsiella pneumoniae strains. The polymyxin-resistant strain Ci, a mutant of Klebsiella pneumoniae ATCC BAA 2146, showed missense mutation in crrB. The crrB mutant Ci, which displayed lipid A modification with 4-amino-4-deoxy-L-arabinose (L-Ara4N) and palmitoylation, showed striking increases of CrrAB, PmrAB, PhoPQ, ArnBCADT and PagP. We hypothesize that crrB mutations induce elevated expression of the arnBCADTEF operon and pagP via PmrAB and PhoPQ. Moreover, multidrug efflux pump KexD, which was induced by crrB mutation, also contributed to colistin resistance. Overall, our results demonstrated proteomic responses to colistin treatment and the mechanism of CrrB-mediate colistin resistance, which may further offer valuable information to manage polymyxin resistance.

2022-04-11 | PXD018528 | Pride

GNPS Palythoa extracts

Project description:LC-MS/MS (ESI negative mode) of ethanol extracts of zoanthids P. caribaeorum and P. variabilis collected at beaches from Ceara, Rio de Janeiro and Santa Catarina States, in Brazil

2016-01-21 | MSV000079467 | GNPS

Project description:SARS-CoV-2 genome sequencing Brazil (Amazonas, Rio Grande do Norte, Paraiba, Bahia and Rio de Janeiro)

| PRJNA752057 | ENA

Infective Endocarditis caused by Enterobacteriaceae: phenotypic and molecular characterization of Escherichia coli and Klebsiella pneumoniae causing endocarditis in Rio de Janeiro, Brazil.

Project description:Characterization of bacterial strains causing endocarditis in hospitalized patients in Rio de Janeiro, Brazil.

| PRJEB37850 | ENA

Bioprospection of the aromatic potential of species from the Atlantic Rainforest in São Paulo: occurrence, taxonomy and chemical, genetic and physiological characterization of plant populations

Project description:Native plants were sampled from nine Atlantic rainforest locations in Brazil for botanical identification, herbarium mounts and chemical analyses. Plants were marked and the coordinate reference determined by Global Positioning System (GPS). Botanical names are as presented in the list of species of Brazilian flora (Lista de Espécies da Flora do Brasil, Jardim Botânico do Rio de Janeiro, 2015). Voucher specimens were deposited at the Herbarium of Instituto Agronômico de Campinas (IAC) (http://herbario.iac.sp.gov.br/), under the given accession numbers (Table 1). Marked plants were resampled in two subsequent years and those failing to be located or in poor condition were excluded from analyses. Environmental data were obtained from meteorological stations at the locations. Monthly values were used to calculate the average seasonal data.

2017-05-14 | ST000606 | MetabolomicsWorkbench

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data