Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human ESC-embryoid body sorted populations: TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi

ABSTRACT: Human ESC-embryoid body sorted populations: TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi

PROVIDER: PRJNA523383 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Human ESC-embryoid body sorted populations: TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi

Project description:Signal differences between TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi in representative cardiac and hepatic gene expression in mRNA microarray analysis. INTRODUCTION: Non-genetic purification methods for pluripotent stem cell-derived hepatocyte-like cells are useful for liver regenerative therapy and pharmaceutical applications. METHODS: Fluorescent activated cell sorting (FACS) was used to separate cells by combining two parameters: cellular mitochondrial content evaluated by the mitochondrial membrane potential-dependent fluorescent probe (TMRM) and immunocytochemical detection of activated leukocyte cell adhesion molecule (ALCAM). This method was applied to murine fetal, human embryonic stem cell (ESC)-derived, and human induced pluripotent stem cell (iPSC)-derived cell-mixtures. Separately sorted cell fractions were evaluated by quantitative PCR, immunohistochemistry, and cytochemistry for HNF4a, AFP, and albumin mRNA and/or protein expression. RESULTS: Hepatocyte-like cells were highly segregated into the high TMRM signal and ALCAM-positive population. The purity of hepatocyte-like cells derived from human iPSCs was 97 ± 0.38% (n = 5). CONCLUSIONS: This hepatocyte-like cell purification method may be applicable to quality control of cells for liver regenerative cell therapy and pharmaceutical development.

2020-03-01 | GSE126812 | GEO

4DNESB7XYI9V - sci-Hi-C on mESCs differentiated to embryoid body

Project description:4DNESB7XYI9V - sci-Hi-C on mESCs differentiated to embryoid body

| PRJNA770075 | ENA

Early embryoid body differentiation

Project description:Embryonic stem (ES) cells, when grown in suspension culture without feeders, spontaneously form round structures known as embryoid bodies. Given the appropriate conditions, these cells can differentiate over time into precursors of all three germ layers. Embryoid bodies, in a disorganized way, mimic early embryonic development to a certain extent and can be used as a synchronously differentiating large scale source of tissue for the study of biological determinants of early differentiation. Embryoid bodies have been used as a source for most early protocols that derive specific differentiated cell types from undifferentiated ES cells, although some protocols, notably those that derive neurons from ES cells, have moved on from EBs as a result of varying replicability and yield. We have decided to look at the transcriptomic profiles of embryoid bodies during the initial stages of embryoid body formation and differentiation, in order to pinpoint novel determinants of key developmental stages. Keywords: time course

2007-08-15 | GSE8766 | GEO

Promoter Capture Hi-C of mouse ESC and TSC

Project description:Chromatin organisation of trophoblast stem cells (TSC) were compared with that of embryonic stem cells (ESC). The method enriches Hi-C libraries, to detect promoter interactions at restriction fragment level. We prepared Hi-C libraries from TSC and ESC (serum grown) samples and enriched them with a promoter capture bait system that captures ~22.000 promoters. Promoter interactions were then analysed using the GOTHiC pipeline.

2018-09-05 | E-MTAB-6585 | biostudies-arrayexpress

Transcription profiling of mouse embryoid body differentation in a time series

Project description:Expression profiling of a 16 day mouse embryoid body (EB) differentiation time course.

2008-06-01 | E-TABM-433 | biostudies-arrayexpress

Expression data from embryoid body with BRCA1 mutation [mRNA]

Project description:We examined the functional significance of the R1699Q variant of human BRCA1 gene using a mouse ES cell-based assay. The total RNA from the embryoid body of WT, R1699Q and M1652I at day 7 were labeled and used for hybridization against Affymetrix gene expression microarray.

2011-12-31 | E-GEOD-31611 | biostudies-arrayexpress

Real-Time PCR analysis of Embryoid Body differentiation of Human pluripotent stem cells

Project description:Human pluripotent stem cells were differentiated by size-controlled embryoid body (EB) formation in defined neutral, ectoderm, endoderm and mesoderm conditions. EB's were analysed at 4, 10 and 16 days post induction by real-time PCR using the Fludigm Deltagene primers and probes.

2017-06-20 | GSE97963 | GEO

Promoter Capture Hi-C of mouse ESC and TSC

Project description:Promoter Capture Hi-C of mouse ESC and TSC

| PRJEB25496 | ENA

4DNESOUYRIO9 - sci-ATAC-seq on mESCs differentiated to embryoid body

Project description:4DNESOUYRIO9 - sci-ATAC-seq on mESCs differentiated to embryoid body

| PRJNA765361 | ENA

4DNESCX7WHJ1 - sci-RNA-seq on mESCs differentiated to embryoid body

Project description:4DNESCX7WHJ1 - sci-RNA-seq on mESCs differentiated to embryoid body

| PRJNA765362 | ENA