Project description:Chromatin enriched by immunopurification with antibodies against the Drosophila transcription factor spotted dick compared with pre immune sera on a cDNA microarray Keywords: parallel sample
Project description:Microplastics (MPs) as widespread contamination pose high risk for aquatic organisms.Intestinal microbiotahas have high interaction with immune system of host body. In this study, intestinal microbiota of zebrafish after Polystyrene (PS-MPs) exposure were characterized by 16S rDNA amplicon sequencing. We found that 100nm and 200μm PS-MPs exposure significantly increased diversity of intestinal microbiota and all the three sizes of PS-MPs increased abundance of pathogenic bacteria.
Project description:Both spotted long oligonucleotide arrays and Affymetrix GeneChips were used to measure differential gene expression in two RNA samples (K562 erythroleukemia RNA and the Stratagene Universal Human Reference RNA). For Affymetrix technology, the two RNAs were analyzed separately. There are two replicates for K562 RNA (GSM4843 and GSM4844) and three for the Stratagene Universal reference (GSM4845-GSM4847). Two types of spotted long oligonucleotide arrays were used. These arrays were used to do two-color hybridizations to directly compare K562 and Universal reference RNAs. One array (GPL273) was made with probes from the Operon Human Genome Oligo Set Version 1. These arrays were used with unamplified cDNA probes (6 replicates, GSM4848-GSM4853), with aRNA probes produced by one round of T7 RNA polymerase-based amplification (6 replicates, GSM4854-GSM4859), and with aRNA probes produced by two rounds of amplification (2 replicates, GSM4860-GSM4861). Keywords: parallel sample
Project description:Drosophila S2 cells treated with either GFP or spottes-dick dsRNA and incubated for 5 days. There are three replicates for each condition. Keywords = Spotted-dick Keywords: other
Project description:Columbia spotted frogs (Rana luteiventris) have characteristic microbiota that may be shaped by their skin peptides and the environment
Project description:In the ex situ conservation of chondrycthyan species, successful reproduction in aquaria is essential. However, aquatic species often exhibit reduced reproductive success under human care. Different factors, including water temperature, nutrition, and intrinsic genetic and epigenetic elements, influence their fertility. Conventional sperm analyses do not provide insights into the functional competence of semen. Therefore, proteomics is gaining relevance, increasing a better understanding of male physiology. In this study, we investigated the proteomic profiles of seminal plasma and spermatozoa from small-spotted catsharks in two different environments: natural environment and aquarium environment.
Project description:Chromatin enriched by immunopurification with antibodies against the Drosophila transcription factor spotted dick compared with pre immune sera on a cDNA microarray
